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Literature summary for 2.3.1.181 extracted from

  • Zhao, X.; Miller, J.R.; Cronan, J.E.
    The reaction of LipB, the octanoyl-[acyl carrier protein]:protein N-octanoyltransferase of lipoic acid synthesis, proceeds through an acyl-enzyme intermediate (2005), Biochemistry, 44, 16737-16746.
    View publication on PubMed
Search for more literature for 2.3.1.181

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant His-tagged LipB proteins Escherichia coli

Protein Variants

Protein Variants Comment Organism
C137A/C169A mutant protein retains trace activity Escherichia coli
C169A mutant enzyme retains trace activity, mutant enzyme forms an octanoyl-LpiB species that is not catalytically competent Escherichia coli
C169S mutant enzyme has no activity, mutant enzyme forms an octanoyl-LpiB species that is not catalytically competent Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
octanoyl-[acyl-carrier protein] + protein Escherichia coli
-
 protein N6-(octanoyl)lysine + acyl-carrier protein
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant His-tagged LipB proteins Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
octanoyl-[acyl-carrier protein] + apo-E2 domain reaction proceeds through an acyl-enzyme intermediate Escherichia coli apo E2-domain protein N6-(octanoyl)lysine + acyl-carrier protein
-
 ?
octanoyl-[acyl-carrier protein] + protein
-
 Escherichia coli protein N6-(octanoyl)lysine + acyl-carrier protein
-
 ?

Synonyms

Synonyms Comment Organism
LipB
-
 Escherichia coli
octanoyl-[acyl carrier protein]:protein N-octanoyltransferase
-
 Escherichia coli