Cloned (Comment) | Organism |
---|---|
gene lipM, complements a lipB-defective Escherichia coli mutant strain | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of strain NM57 in which lipM is replaced with a kanamycin-resistance determinant. Gene lipM is placed under control of a xylose-inducible promoter PxylA is introduced into the DELTAlipM strain NM57 giving the lipM amyE::PxylA-lipM strain NM08. LipM can be functionally replaced by expression of Escherichia coli lipB, expression of Escherichia coli lipB allows growth of Bacillus subtilis DELTAlipL or DELATlipM strains in the absence of supplements. In contrast, growth of an Escherichia coli DELTAlipB strain can be complemented with lipM, but not lipL | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
an octanoyl-[acyl-carrier protein] + a protein | Bacillus subtilis | - |
a protein N6-(octanoyl)lysine + an [acyl-carrier protein] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
gene lipM | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
an octanoyl-[acyl-carrier protein] + a protein | - |
Bacillus subtilis | a protein N6-(octanoyl)lysine + an [acyl-carrier protein] | - |
? | |
an octanoyl-[acyl-carrier protein] + a protein | acyl-enzyme intermediate mechanism | Bacillus subtilis | a protein N6-(octanoyl)lysine + an [acyl-carrier protein] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ACP:protein-N-octanoyltransferase | - |
Bacillus subtilis |
LipM | - |
Bacillus subtilis |
octanoyl-acyl carrier protein:protein-N-octanoyltransferase | - |
Bacillus subtilis |
General Information | Comment | Organism |
---|---|---|
malfunction | DELTAlipL strains are unable to synthesize lipoic acid despite the presence of LipM and the sulfur insertion enzyme, LipA, which should suffice for lipoic acid biosynthesis. Strain NM57 DELTAlipM is auxotrophic for lipoic acid when grown in minimal medium but grew as well as the wild type strain JH642 in the presence of lipoic acid | Bacillus subtilis |
physiological function | LipM is required for modification of lipoyl domains by the biosynthetic pathway, it is responsible for octanoyl transfer in vivo requirement for LipL. But LipM is only required for the endogenous lipoylation pathway, whereas LipL also plays a role in lipoic acid scavenging | Bacillus subtilis |