Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.184 extracted from

  • Schaefer, A.L.; Val, D.L.; Hanzelka, B.L.; Cronan, J.E., Jr.; Greenberg, E.P.
    Generation of cell-to-cell signals in quorum sensing: acyl homoserine lactone synthase activity of a purified Vibrio fischeri LuxI protein (1996), Proc. Natl. Acad. Sci. USA, 93, 9505-9509.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.184

Cloned(Commentary)

Cloned (Comment) Organism
gene luxI, expression as maltose-binding protein-fusion enzyme in Escherichia coli strain XL 1-Blue Aliivibrio fischeri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0096
-
 hexanoyl-[acyl-carrier protein] pH 7.5, 23°C Aliivibrio fischeri
0.13
-
 S-adenosyl-L-methionine pH 7.5, 23°C Aliivibrio fischeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Aliivibrio fischeri acylhomoserine lactones are autoinducers of quorum-sensing signaling ?
-
 ?
S-adenosyl-L-methionine + 3-oxohexanoyl-[acyl-carrier protein] Aliivibrio fischeri
-
 5'-methylthioadenosine + N-3-oxohexanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?
S-adenosyl-L-methionine + hexanoyl-[acyl-carrier protein] Aliivibrio fischeri
-
 5'-methylthioadenosine + N-hexanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?

Organism

Organism UniProt Comment Textmining
Aliivibrio fischeri
-
 gene luxI
-

Purification (Commentary)

Purification (Comment) Organism
recombinant maltose-binding protein-fusion enzyme from Escherichia coli strain XL 1-Blue by amylose affinity chromatography Aliivibrio fischeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information acylhomoserine lactones are autoinducers of quorum-sensing signaling Aliivibrio fischeri ?
-
 ?
additional information substrate specificity of purified recombinant enzyme Aliivibrio fischeri ?
-
 ?
S-adenosyl-L-methionine + 3-oxohexanoyl-[acyl-carrier protein]
-
 Aliivibrio fischeri 5'-methylthioadenosine + N-3-oxohexanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?
S-adenosyl-L-methionine + hexanoyl-[acyl-carrier protein]
-
 Aliivibrio fischeri 5'-methylthioadenosine + N-hexanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?

Synonyms

Synonyms Comment Organism
acyl homoserine lactone synthase
-
 Aliivibrio fischeri
autoinducer synthase
-
 Aliivibrio fischeri
LuxI protein
-
 Aliivibrio fischeri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20 30
-
 Aliivibrio fischeri

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
18 37 57% of maximal activity at 18°C and 10% at 37°C Aliivibrio fischeri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Aliivibrio fischeri

pH Range

pH Minimum pH Maximum Comment Organism
7.1 8 10% of maximal acivity at pH 7.0, 65% at pH 7.1, and 50% at pH 8.0 Aliivibrio fischeri