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Literature summary for 2.3.1.184 extracted from

  • Parsek, M.R.; Val, D.L.; Hanzelka, B.L.; Cronan, J.E.; Greenberg, E.P.
    Acyl homoserine-lactone quorum-sensing signal generation (1999), Proc. Natl. Acad. Sci. USA, 96, 4360-4365.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.184

Cloned(Commentary)

Cloned (Comment) Organism
gene rhlI, expression in Escherichia coli strain XL 1-Blue Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
5'-methylthioadenosine 67% inhibition at 0.05 mM, 91% inhibition at 0.5 mM Pseudomonas aeruginosa
butyryl-S-adenosyl-L-methionine 24% inhibition at 0.05 mM, 65% inhibition at 0.5 mM Pseudomonas aeruginosa
additional information no inhibition by N-butyryl-L-homoserine-1,4-lactone, CoA, NADH, L-methionine, L-homocysteine, L-homoserine-1,4-lactone, pantothenate, L-homoserine, butyric acid, cerulenin, ATP, ADP, 4-hydroxybutyrate, butyryl-CoA, and apo-ACP Pseudomonas aeruginosa
S-Adenosyl-D-homocysteine 43% inhibition at 0.05 mM, 88% inhibition at 0.5 mM Pseudomonas aeruginosa
S-adenosyl-L-cysteine 77% inhibition at 0.05 mM, 97% inhibition at 0.5 mM Pseudomonas aeruginosa
S-adenosyl-L-homocysteine 62% inhibition at 0.05 mM, 91% inhibition at 0.5 mM Pseudomonas aeruginosa
sinefungin 58% inhibition at 0.1 mM Pseudomonas aeruginosa
[Acyl-carrier-protein] holo-[acyl-carrier-protein], 2% inhibition at 0.05 mM, 55% inhibition at 0.5 mM Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information reaction kinetics of recombinant RhlI Pseudomonas aeruginosa
0.006
-
 butyryl-[acyl-carrier protein] pH 7.8, 37°C, recombinant RhlI Pseudomonas aeruginosa
0.008
-
 hexanoyl-[acyl-carrier protein] pH 7.8, 37°C, recombinant RhlI Pseudomonas aeruginosa
0.014
-
 S-adenosyl-L-methionine pH 7.8, 37°C, recombinant RhlI Pseudomonas aeruginosa
0.038
-
 butyryl-S-adenosyl-L-methionine pH 7.8, 37°C, recombinant RhlI Pseudomonas aeruginosa
0.043
-
 octanoyl-[acyl-carrier protein] pH 7.8, 37°C, recombinant RhlI Pseudomonas aeruginosa
0.23
-
 butyryl-CoA pH 7.8, 37°C, recombinant RhlI Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas aeruginosa the enzyme is involved in quorum-sensing signaling by synthesizing the signaling molecules which regulates the virulence genes, N-acylhomoserine lactones are autoinducers, the enzyme acts as a quorum-sensing signal generator, mechanism, overview ?
-
 ?
additional information Pseudomonas aeruginosa PAO-JP1 the enzyme is involved in quorum-sensing signaling by synthesizing the signaling molecules which regulates the virulence genes, N-acylhomoserine lactones are autoinducers, the enzyme acts as a quorum-sensing signal generator, mechanism, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
 gene rhlI
-
Pseudomonas aeruginosa PAO-JP1
-
 gene rhlI
-

Purification (Commentary)

Purification (Comment) Organism
recombinant RhlI 95fold from Escherichia coli strain XL 1-Blue by anion and cation exchange chromatography and gel filtration Pseudomonas aeruginosa

Reaction

Reaction Comment Organism Reaction ID
an acyl-[acyl-carrier protein] + S-adenosyl-L-methionine = an [acyl-carrier protein] + S-methyl-5'-thioadenosine + an N-acyl-L-homoserine lactone RhlI shows a sequential, ordered ping-pong reaction mechanism with S-adenosyl-L-methionine binding first Pseudomonas aeruginosa
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0021
-
 purified recombinant RhlI Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is involved in quorum-sensing signaling by synthesizing the signaling molecules which regulates the virulence genes, N-acylhomoserine lactones are autoinducers, the enzyme acts as a quorum-sensing signal generator, mechanism, overview Pseudomonas aeruginosa ?
-
 ?
additional information substrate specificity of RhlI, no activity with butyrate, hexanoyl-CoA, and decanoyl-[acyl-carrier-protein], no activity with S-adenosyl-L-homocysteine, S-adenosyl-L-cysteine, L-homoserine lactone, L-homocystein, L-homoserine, and L-methionine as substrates Pseudomonas aeruginosa ?
-
 ?
additional information the enzyme is involved in quorum-sensing signaling by synthesizing the signaling molecules which regulates the virulence genes, N-acylhomoserine lactones are autoinducers, the enzyme acts as a quorum-sensing signal generator, mechanism, overview Pseudomonas aeruginosa PAO-JP1 ?
-
 ?
additional information substrate specificity of RhlI, no activity with butyrate, hexanoyl-CoA, and decanoyl-[acyl-carrier-protein], no activity with S-adenosyl-L-homocysteine, S-adenosyl-L-cysteine, L-homoserine lactone, L-homocystein, L-homoserine, and L-methionine as substrates Pseudomonas aeruginosa PAO-JP1 ?
-
 ?
S-adenosyl-L-methionine + butyryl-CoA
-
 Pseudomonas aeruginosa 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + CoA
-
 ?
S-adenosyl-L-methionine + butyryl-S-adenosyl-L-methionine
-
 Pseudomonas aeruginosa 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + S-adenosyl-L-methionine
-
 ?
S-adenosyl-L-methionine + butyryl-[acyl-carrier protein]
-
 Pseudomonas aeruginosa 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?
S-adenosyl-L-methionine + butyryl-[acyl-carrier protein]
-
 Pseudomonas aeruginosa PAO-JP1 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?
S-adenosyl-L-methionine + hexanoyl-[acyl-carrier protein]
-
 Pseudomonas aeruginosa 5'-methylthioadenosine + N-hexanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?
S-adenosyl-L-methionine + hexanoyl-[acyl-carrier protein]
-
 Pseudomonas aeruginosa PAO-JP1 5'-methylthioadenosine + N-hexanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?
S-adenosyl-L-methionine + octanoyl-[acyl-carrier protein]
-
 Pseudomonas aeruginosa 5'-methylthioadenosine + N-octanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?
S-adenosyl-L-methionine + octanoyl-[acyl-carrier protein]
-
 Pseudomonas aeruginosa PAO-JP1 5'-methylthioadenosine + N-octanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?

Synonyms

Synonyms Comment Organism
acyl homoserine lactone synthase
-
 Pseudomonas aeruginosa
More the enzyme belongs to the LuxI family Pseudomonas aeruginosa
RhlI
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Pseudomonas aeruginosa