Literature summary for 2.3.1.191 extracted from

Badger, J.; Chie-Leon, B.; Logan, C.; Sridhar, V.; Sankaran, B.; Zwart, P.H.; Nienaber, V.
The structure of LpxD from Pseudomonas aeruginosa at 1.3 A resolution (2011), Acta Crystallogr. Sect. F, 67, 749-752.

Search for more literature for 2.3.1.191

Cloned(Commentary)

Cloned (Comment)	Organism
gene lpxD, expression of N-terminally His6-tagged LpxD in Escherichia coli strain BL21 (DE3)	Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant His6-tagged LpxD, hanging drop vapour diffusion, 0.002 ml of 15 mg/ml LpxD in 10 mM Tris pH 8, 500 mM NaCl and 1 mM DTT, with 50 mM uridine diphosphate-N-acetylglucosamine, is mixed with 0.002 ml of 100 mM Tris pH 8.5, 1.5 M lithium sulfate, 20°C, 3-5 days, X-ray diffraction structure determination and analysis at 1.3 A resolution, molecular replacement	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	Q9HXY6	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant N-terminally His6-tagged LpxD from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and gel filtration	Pseudomonas aeruginosa

Subunits

Subunits	Comment	Organism
More	the LpxD domain architecture is consisting of an N-terminal domain, amino acids 2-95, a left-handed beta-helix central domain containing many short parallel beta-sheet segments wrappingthrough consecutive triangular sections, amino acids 101-305, and a C-terminal helix, amino acids 312-340	Pseudomonas aeruginosa

Synonyms

Synonyms	Comment	Organism
LpxD	-	Pseudomonas aeruginosa

General Information

General Information	Comment	Organism
physiological function	LpxD is part of the biosynthesis pathway of lipid A and is responsible for transferring 3-hydroxymyristic acid from the R-3-hydroxymyristoyl-acyl carrier protein to the 2-OH group of UDP-3-O-(3-hydroxymyristoyl) glucosamine. The first three enzymes responsible for Gram-negative bacterial cell-wall synthesis, LpxA, LpxC and LpxD, are all present as single copies and are essential for bacterial viability	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)