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Literature summary for 2.3.1.199 extracted from
- Two modes of regulation of the fatty acid elongase ELOVL6 by the 3-ketoacyl-CoA reductase KAR in the fatty acid elongation cycle (2014), PLoS ONE, 9, e101823.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the 3-ketoacyl-CoA reductase, KAR, the enzyme catalyzing the next step in metabolism after fatty acid elongase ELOVL6, largely stimulates ELOVL6 activity. Activity of purified enzyme ELOVL6 is enhanced by about 3fold in the presence of 3-ketoacyl-CoA reductase, KAR. This effect is KAR enzyme activity-independent, since it is observed in the absence of NADPH and in the poorly active or inactive KAR mutants, overview. ELOVL6 enzyme activity is also enhanced in a KAR enzyme activity-dependent manner | Homo sapiens | |
| NADPH | ELOVL6 activity in membrane fractions is enhanced in the presence of NADPH | Homo sapiens |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of N-terminally 3xFLAG-tagged enzyme ELOVL6 in HEK-293 T cells | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00122 | - |
palmitoyl-CoA | pH 6.8, 37°C, recombinant enzyme | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Homo sapiens | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
x * 29000, recombinant deglycosylated 3xFLAG-tagged enzyme, SDS-PAGE, x * 32000, recombinant glycosylated 3xFLAG-tagged enzyme, SDS-PAGE | Homo sapiens |
| 32000 | - |
x * 29000, recombinant deglycosylated 3xFLAG-tagged enzyme, SDS-PAGE, x * 32000, recombinant glycosylated 3xFLAG-tagged enzyme, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| a very-long-chain acyl-CoA + malonyl-CoA | Homo sapiens | - |
a very-long-chain 3-oxoacyl-CoA + CO2 + coenzyme A | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q9H5J4 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | deglycosylation is possibel with Endo H | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-terminally 3xFLAG-tagged enzyme ELOVL6 from HEK-293 T cells by solubilization with Triton X-100 and affinity chromatography | Homo sapiens |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| recombinant enzyme ELOVL6 has no enzyme activity under solubilized conditions, reconstitution of ELOVL6 into proteoliposomes enables it to exhibit enzyme activity | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| a very-long-chain acyl-CoA + malonyl-CoA | - |
Homo sapiens | a very-long-chain 3-oxoacyl-CoA + CO2 + coenzyme A | - |
? | |
| additional information | no activity with saturated and unsaturated fatty acids longer than C16 | Homo sapiens | ? | - |
? | |
| palmitoyl-CoA + malonyl-CoA | - |
Homo sapiens | 3-oxostearoyl-CoA + CO2 + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 29000, recombinant deglycosylated 3xFLAG-tagged enzyme, SDS-PAGE, x * 32000, recombinant glycosylated 3xFLAG-tagged enzyme, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Elovl6 | - |
Homo sapiens |
| FA elongase | - |
Homo sapiens |
| Fatty acid elongase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
assay at | Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme is involved in the fatty acid elongation cycle consisting of the reaction steps of condensation, reduction, dehydration, and reduction | Homo sapiens |
| physiological function | 3-ketoacyl-CoA reductase, KAR, regulates enzyme ELOVL6 via two modes: in the first mode, KAR may induce conformational changes in ELOVL6 to become a structure that can undergo catalysis. In the second mode, conversion of 3-oxoacyl-CoA to 3-hydroxyacyl-CoA by KAR may facilitate release of the product from the presumed ELOVL6-KAR complex | Homo sapiens |
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