Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli C41(DE3) cells | Marinobacter nauticus |
Protein Variants | Comment | Organism |
---|---|---|
D145A | the mutant is 5fold less active with 1,2-dipalmitoyl-sn-glycerol compared to the wild type enzyme | Marinobacter nauticus |
D271A | the mutant shows reduced activity compared to the wild type enzyme | Marinobacter nauticus |
H140A | the mutant is 10fold less active with 1,2-dipalmitoyl-sn-glycerol compared to the wild type enzyme | Marinobacter nauticus |
H141A | the mutant is 5fold less active with 1,2-dipalmitoyl-sn-glycerol compared to the wild type enzyme | Marinobacter nauticus |
L119A | the mutant shows reduced activity compared to the wild type enzyme | Marinobacter nauticus |
N270A | the mutant shows reduced activity compared to the wild type enzyme | Marinobacter nauticus |
R305A | the mutant shows reduced activity compared to the wild type enzyme | Marinobacter nauticus |
W120A | inactive | Marinobacter nauticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
x * 100000, SDS-PAGE | Marinobacter nauticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Marinobacter nauticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and S200 gel filtration | Marinobacter nauticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme shows very low activity with ethanol, methanol, butanol, and glycerol | Marinobacter nauticus | ? | - |
? | |
palmitoyl-CoA + 1,2-dipalmitoyl-sn-glycerol | - |
Marinobacter nauticus | CoA + tripalmitoylglycerol | - |
? | |
palmitoyl-CoA + hexadecanol | - |
Marinobacter nauticus | hexadecyl palmitate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 100000, SDS-PAGE | Marinobacter nauticus |
Synonyms | Comment | Organism |
---|---|---|
acyl coenzyme A:diacylglycerol acyltransferase | - |
Marinobacter nauticus |
Ma2 | - |
Marinobacter nauticus |