Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae strain InvSc1 | Brassica napus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
2 * 58000, SDS-PAGE | Brassica napus |
64000 | - |
2 * 64000, calculated from amino acid sequence | Brassica napus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica napus | K9LLA9 | cultivar Jet Neuf | - |
Purification (Comment) | Organism |
---|---|
solubilization in n-dodecyl-beta-D-maltopyranoside, cobalt affinity chromatography, and Superdex 200 gel filtration | Brassica napus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00341 | - |
crude enzyme, at pH 7.4 and 30°C | Brassica napus |
0.026 | - |
after 126fold purification, at pH 7.4 and 30°C | Brassica napus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-linolenoyl-CoA + 1,2-dioleoyl-sn-glycerol | 100% activity | Brassica napus | CoA + 1,2-dioleoyl-3-linolenoyl-sn-glycerol | - |
? | |
linoleoyl-CoA + 1,2-dioleoyl-sn-glycerol | - |
Brassica napus | CoA + 1,2-dioleoyl-3-linoleoyl-sn-glycerol | - |
? | |
oleoyl-CoA + 1,2-dioleoyl-sn-glycerol | about 85% activity compared to alpha-linolenoyl-CoA | Brassica napus | CoA + 1,2,3-trioleoyl-sn-glycerol | - |
? | |
palmitoyl-CoA + 1,2-dioleoyl-sn-glycerol | about 85% activity compared to alpha-linolenoyl-CoA | Brassica napus | CoA + 1,2-dioleoyl-3-palmitoyl-sn-glycerol | - |
? | |
stearoyl-CoA + 1,2-dioleoyl-sn-glycerol | - |
Brassica napus | CoA + 1,2-dioleoyl-3-stearoyl-sn-glycerol | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 58000, SDS-PAGE | Brassica napus |
homodimer | 2 * 64000, calculated from amino acid sequence | Brassica napus |
Synonyms | Comment | Organism |
---|---|---|
DGAT1 | - |
Brassica napus |
diacylglycerol acyltransferase 1 | - |
Brassica napus |