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Literature summary for 2.3.1.20 extracted from

  • Caldo, K.M.; Greer, M.S.; Chen, G.; Lemieux, M.J.; Weselake, R.J.
    Purification and properties of recombinant Brassica napus diacylglycerol acyltransferase 1 (2015), FEBS Lett., 589, 773-778.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain InvSc1 Brassica napus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
2 * 58000, SDS-PAGE Brassica napus
64000
-
2 * 64000, calculated from amino acid sequence Brassica napus

Organism

Organism UniProt Comment Textmining
Brassica napus K9LLA9 cultivar Jet Neuf
-

Purification (Commentary)

Purification (Comment) Organism
solubilization in n-dodecyl-beta-D-maltopyranoside, cobalt affinity chromatography, and Superdex 200 gel filtration Brassica napus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00341
-
crude enzyme, at pH 7.4 and 30°C Brassica napus
0.026
-
after 126fold purification, at pH 7.4 and 30°C Brassica napus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-linolenoyl-CoA + 1,2-dioleoyl-sn-glycerol 100% activity Brassica napus CoA + 1,2-dioleoyl-3-linolenoyl-sn-glycerol
-
?
linoleoyl-CoA + 1,2-dioleoyl-sn-glycerol
-
Brassica napus CoA + 1,2-dioleoyl-3-linoleoyl-sn-glycerol
-
?
oleoyl-CoA + 1,2-dioleoyl-sn-glycerol about 85% activity compared to alpha-linolenoyl-CoA Brassica napus CoA + 1,2,3-trioleoyl-sn-glycerol
-
?
palmitoyl-CoA + 1,2-dioleoyl-sn-glycerol about 85% activity compared to alpha-linolenoyl-CoA Brassica napus CoA + 1,2-dioleoyl-3-palmitoyl-sn-glycerol
-
?
stearoyl-CoA + 1,2-dioleoyl-sn-glycerol
-
Brassica napus CoA + 1,2-dioleoyl-3-stearoyl-sn-glycerol
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 58000, SDS-PAGE Brassica napus
homodimer 2 * 64000, calculated from amino acid sequence Brassica napus

Synonyms

Synonyms Comment Organism
DGAT1
-
Brassica napus
diacylglycerol acyltransferase 1
-
Brassica napus