Cloned (Comment) | Organism |
---|---|
cloning and expression of CPT II in Escherichia coli, overexpression of CPT II in Spodoptera frugiperda Sf 9 cells via baculavirus infection, DNA sequence analysis | Rattus norvegicus |
General Stability | Organism |
---|---|
no loss in activity after 6 freeze/thaw cycles at -70°C or room temeprature, recombinant CPT II | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.034 | - |
palmitoyl-CoA | recombinant CPT II | Rattus norvegicus | |
0.95 | - |
L-carnitine | recombinant CPT II | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + L-carnitine | Rattus norvegicus | - |
CoA + L-acylcarnitine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
enzyme form CPT II | - |
Purification (Comment) | Organism |
---|---|
recombinant CPT II from Sf 9 insect cells | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | CPT II | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.43 | - |
recombinant CPT II from Sf 9 insect cells | Rattus norvegicus |
Storage Stability | Organism |
---|---|
4°C, pH 7.0-10.0, 24 h stable, recombinant CPT II | Rattus norvegicus |
room temperature, 6.5 h, no loss in activity, recombinant CPT II | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + L-carnitine | - |
Rattus norvegicus | CoA + L-acylcarnitine | - |
? | |
palmitoyl-CoA + L-carnitine | - |
Rattus norvegicus | CoA + L-palmitoylcarnitine | - |
? |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | stable for 24 h at 4°C, recombinant CPT II | Rattus norvegicus |