Cloned (Comment) | Organism |
---|---|
2 isoforms of CPT I, liver L-CPT I, skeletal muscle M-CPT I, cloned from liver, expression in Pichia pastoris GS115, DNA sequence determination | Sus scrofa |
in vitro transcription and translation of L-CPT I | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
malonyl-CoA | L-CPT I | Sus scrofa | |
additional information | L-CPT I behaves like a natural chimera of M-CPT I and L-CPT I due to malonyl-CoA sensitivity and Km values compared to the enzymes of other organisms | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | L-CPT I behaves like a natural chimera of M-CPT I and L-CPT I due to malonyl-CoA sensitivity and Km values compared to the enzymes of other organisms | Sus scrofa | |
0.035 | - |
palmitoyl-CoA | recombinant wild-type L-CPT I | Sus scrofa | |
0.126 | - |
L-carnitine | recombinant wild-type L-CPT I | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial outer membrane | CPT I is located in the outer membrane | Sus scrofa | 5741 | - |
mitochondrion | 2 isoforms of CPT I, L-CPT I and M-CPT I | Sus scrofa | 5739 | - |
mitochondrion | recombinant L-CPT I and M-CPT I in Pichia pastoris | Sus scrofa | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
82000 | - |
x * 82000, L-CPT I, SDS-PAGE | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + L-carnitine | Sus scrofa | - |
CoA + L-acylcarnitine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
2 isoforms of CPT I in liver mitochondria, liver L-CPT I, skeletal muscle M-CPT I | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
duodenum | L-CPT I | Sus scrofa | - |
heart | L-CPT I | Sus scrofa | - |
heart | M-CPT I | Sus scrofa | - |
kidney | L-CPT I | Sus scrofa | - |
liver | L-CPT I | Sus scrofa | - |
liver | M-CPT I | Sus scrofa | - |
liver | CPT I | Sus scrofa | - |
skeletal muscle | M-CPT I | Sus scrofa | - |
testis | L-CPT I, not M-CPT I | Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + L-carnitine | - |
Sus scrofa | CoA + L-acylcarnitine | - |
? | |
palmitoyl-CoA + L-carnitine | - |
Sus scrofa | CoA + L-palmitoylcarnitine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 82000, L-CPT I, SDS-PAGE | Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Sus scrofa |