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Literature summary for 2.3.1.21 extracted from

  • Napal, L.; Dai, J.; Treber, M.; Haro, D.; Marrero, P.F.; Woldegiorgis, G.
    A single amino acid change (substitution of the conserved Glu-590 with alanine) in the C-terminal domain of rat liver carnitine palmitoyltransferase I increases its malonyl-CoA sensitivity close to that observed with the muscle isoform of the enzyme (2003), J. Biol. Chem., 278, 34084-34089.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
E590A IC50 for malonyl CoA is 16fold lower than the wild-type value, partial decrease in catalytic activity,1.5fold increase in Km-value for carnitine, 2.9fold decrease in KM-value doe palmitoyl-CoA Rattus norvegicus
E590D inactive mutant enzyme Rattus norvegicus
E590K IC50 for malonyl CoA is 13.5fold lower than the wild-type value, partial decrease in catalytic activity Rattus norvegicus
E590Q IC50 for malonyl CoA is 8.7fold lower than the wild-type value, partial decrease in catalytic activity, 1.3fold increase in Km-value for carnitine, 2fold decrease in KM-value doe palmitoyl-CoA Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
malonyl-CoA IC50: 0.00339 mM for wild-type enzyme, 0.00021 mM for mutant enzyme E590A, 0.00039 mM for mutant enzyme E590Q, 0.00025 mM for mutant enzyme E590K Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0593
-
L-carnitine wild-type enzyme Rattus norvegicus
0.0622
-
palmitoyl-CoA mutant enzyme E590A Rattus norvegicus
0.0744
-
palmitoyl-CoA mutant enzyme E590Q Rattus norvegicus
0.0748
-
L-carnitine mutant enzyme E590Q Rattus norvegicus
0.0876
-
L-carnitine mutant enzyme E590A Rattus norvegicus
0.1535
-
palmitoyl-CoA wild-type enzyme Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Rattus norvegicus 5739
-

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P32198
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
palmitoyl-CoA + L-carnitine
-
Rattus norvegicus CoA + L-palmitoylcarnitine
-
?

Synonyms

Synonyms Comment Organism
carnitine palmitoyltransferase I
-
Rattus norvegicus
CPTi
-
Rattus norvegicus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00339
-
IC50: 0.00339 mM for wild-type enzyme, 0.00021 mM for mutant enzyme E590A, 0.00039 mM for mutant enzyme E590Q, 0.00025 mM for mutant enzyme E590K Rattus norvegicus malonyl-CoA