Protein Variants | Comment | Organism |
---|---|---|
E590A | IC50 for malonyl CoA is 16fold lower than the wild-type value, partial decrease in catalytic activity,1.5fold increase in Km-value for carnitine, 2.9fold decrease in KM-value doe palmitoyl-CoA | Rattus norvegicus |
E590D | inactive mutant enzyme | Rattus norvegicus |
E590K | IC50 for malonyl CoA is 13.5fold lower than the wild-type value, partial decrease in catalytic activity | Rattus norvegicus |
E590Q | IC50 for malonyl CoA is 8.7fold lower than the wild-type value, partial decrease in catalytic activity, 1.3fold increase in Km-value for carnitine, 2fold decrease in KM-value doe palmitoyl-CoA | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
malonyl-CoA | IC50: 0.00339 mM for wild-type enzyme, 0.00021 mM for mutant enzyme E590A, 0.00039 mM for mutant enzyme E590Q, 0.00025 mM for mutant enzyme E590K | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0593 | - |
L-carnitine | wild-type enzyme | Rattus norvegicus | |
0.0622 | - |
palmitoyl-CoA | mutant enzyme E590A | Rattus norvegicus | |
0.0744 | - |
palmitoyl-CoA | mutant enzyme E590Q | Rattus norvegicus | |
0.0748 | - |
L-carnitine | mutant enzyme E590Q | Rattus norvegicus | |
0.0876 | - |
L-carnitine | mutant enzyme E590A | Rattus norvegicus | |
0.1535 | - |
palmitoyl-CoA | wild-type enzyme | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P32198 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-CoA + L-carnitine | - |
Rattus norvegicus | CoA + L-palmitoylcarnitine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
carnitine palmitoyltransferase I | - |
Rattus norvegicus |
CPTi | - |
Rattus norvegicus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00339 | - |
IC50: 0.00339 mM for wild-type enzyme, 0.00021 mM for mutant enzyme E590A, 0.00039 mM for mutant enzyme E590Q, 0.00025 mM for mutant enzyme E590K | Rattus norvegicus | malonyl-CoA |