Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, phylogenetic tree, expression of His-tagged wild-type and mutant enzymes in Escherichia coli | Rheum palmatum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
4-coumaroyl-CoA | pH 8.0, 30°C, recombinant wild-type enzyme | Rheum palmatum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42223 | - |
x * 42223, sequence calculation | Rheum palmatum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + malonyl-CoA + H2O | Rheum palmatum | - |
2 CoA + 4-hydroxybenzalacetone + 2 CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rheum palmatum | Q94FV7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography and gel filtration | Rheum palmatum |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + malonyl-CoA + H2O | - |
Rheum palmatum | 2 CoA + 4-hydroxybenzalacetone + 2 CO2 | - |
? | |
additional information | the recombinant enzyme expressed in Escherichia coli efficiently affords benzalacetone as a single product from 4-coumaroyl-CoA and malonyl-CoA. BAS does not accept hexanoyl-CoA, isobutyryl-CoA, isovaleryl-CoA, and acetyl-CoA as a substrates. No conversion of 3-(4-hydroxyphenyl)propionyl-CoA to 4-(4-hydroxyphenyl)butan-2-one | Rheum palmatum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42223, sequence calculation | Rheum palmatum |
Synonyms | Comment | Organism |
---|---|---|
bAS | - |
Rheum palmatum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rheum palmatum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0298 | - |
4-coumaroyl-CoA | pH 8.0, 30°C, recombinant wild-type enzyme | Rheum palmatum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.8 | - |
Rheum palmatum |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a plant-specific type III polyketide synthase of the chalcone synthase (CHS) superfamily. Members of the CHS-superfamily enzymes do not form a species-specific cluster, but instead group into subfamilies according to their enzymatic function. BAS lacks the active-site Phe215 residue (numbering in CHS), which has been proposed to help orient substrates and intermediates during the sequential condensation of 4-coumaroyl-CoA with malonyl-CoA in CHS, while the catalytic cysteine-histidine dyad (Cys164-His303) in CHS is well conserved in BAS | Rheum palmatum |
physiological function | BAS plays a crucial role in the biosynthesis of pharmaceutically important phenylbutanone glucoside, lindleyin, the active principle of the anti-inflammatory action of the medicinal plant, and it plays a crucial role for the construction of the C6-C4 moiety of a variety of natural products such as medicinally important gingerols in ginger plant | Rheum palmatum |