Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.225 extracted from

  • Fernandez-Hernando, C.; Fukata, M.; Bernatchez, P. N.; Fukata, Y.; Lin, M. I.; Bredt, D. S.; Sessa, W. C.
    Identification of Golgi-localized acyl transferases that palmitoylate and regulate endothelial nitric oxide synthase (2006), J. Cell. Biol., 174, 369-377.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
COS-7 cells are cotransfected with the cDNAs for eNOS and HA-tagged DHHC-21 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi membrane isozymes DHHC-2, -3, -7, -8, and -21 Homo sapiens 139
-
additional information the isozymes colocalize with eNOS in the Golgi and plasma membrane and interact with eNOS in endothelial cells Homo sapiens
-
-
plasma membrane isozymes DHHC-2, -3, -7, -8, and -21 Homo sapiens 5886
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
palmitoyl-CoA + [endothelial nitric oxide synthase]-L-cysteine Homo sapiens isozyme DHHC-21 [endothelial nitric oxide synthase]-S-palmitoyl-L-cysteine + CoA
-
r
palmitoyl-CoA + [protein]-L-cysteine Homo sapiens
-
[protein]-S-palmitoyl-L-cysteine + CoA
-
r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
endothelial cell expression of 5 PAT isozymes Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
palmitoyl-CoA + [endothelial nitric oxide synthase]-L-cysteine isozyme DHHC-21 Homo sapiens [endothelial nitric oxide synthase]-S-palmitoyl-L-cysteine + CoA
-
r
palmitoyl-CoA + [protein]-L-cysteine
-
Homo sapiens [protein]-S-palmitoyl-L-cysteine + CoA
-
r

Synonyms

Synonyms Comment Organism
Asp-His-His-Cys motif palmitoyl transferase
-
Homo sapiens
DHHC palmitoyl transferase
-
Homo sapiens
DHHC-2
-
Homo sapiens
DHHC-21
-
Homo sapiens
DHHC-21 palmitoyl transferase
-
Homo sapiens
DHHC-3
-
Homo sapiens
DHHC-3 palmitoyl transferase
-
Homo sapiens
DHHC-7
-
Homo sapiens
DHHC-8
-
Homo sapiens
Pat
-
Homo sapiens

General Information

General Information Comment Organism
evolution the enzyme is an Asp-His-His-Cys motif (DHHC) palmitoyl transferase family member Homo sapiens
malfunction palmitoylation of wild-type eNOS by DHHC-21 is diminished by mutation of the two sites of eNOS palmitoylation, cysteines 15 and 26, palmitoylation deficient mutants of eNOS, i.e. G2A, C15/26S, and L2S, release less nitric oxide. Inhibition of DHHC-21 palmitoyl transferase, but not DHHC-3, in human endothelial cells reduces eNOS palmitoylation, eNOS targeting, and stimulated NO production Homo sapiens
physiological function regulatory role of DHHC-21 in governing eNOS localization and function. eNOS fatty acylation is required for an efficient interaction with DHHC proteins and NO release, overview Homo sapiens