Cloned (Comment) | Organism |
---|---|
24 PAT genes from Arabidopsis thaliana, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic tree, expression of GFP-tagged PATs in yeast mutant cells | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | complementation of the Vac8 protein localization to the vacuolar membrane in mutant yeast cells by Arabidopsis PATs | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Arabidopsis thaliana | 5783 | - |
endosome | - |
Arabidopsis thaliana | 5768 | - |
Golgi membrane | - |
Arabidopsis thaliana | 139 | - |
membrane | PATs are transmembrane proteins | Arabidopsis thaliana | 16020 | - |
additional information | Arabidopsis PAT proteins display a complex targeting pattern and are detected at the endoplasmic reticulum, Golgi, endosomal compartments, and the vacuolar membrane, but most proteins are targeted to the plasma membrane | Arabidopsis thaliana | - |
- |
plasma membrane | - |
Arabidopsis thaliana | 5886 | - |
vacuolar membrane | - |
Arabidopsis thaliana | 5774 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-CoA + [protein]-L-cysteine | Arabidopsis thaliana | - |
[protein]-S-palmitoyl-L-cysteine + CoA | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | B3DN87 | 24 PAT family members | - |
Arabidopsis thaliana | O80685 | 24 PAT family members | - |
Arabidopsis thaliana | Q0WQK2 | 24 PAT family members | - |
Arabidopsis thaliana | Q3EBC2 | 24 PAT family members | - |
Arabidopsis thaliana | Q3EC11 | 24 PAT family members | - |
Arabidopsis thaliana | Q500Z2 | 24 PAT family members | - |
Arabidopsis thaliana | Q52T38 | 24 PAT family members | - |
Arabidopsis thaliana | Q5M757 | 24 PAT family members | - |
Arabidopsis thaliana | Q6DR03 | 24 PAT family members | - |
Arabidopsis thaliana | Q7XA86 | 24 PAT family members | - |
Arabidopsis thaliana | Q8L5Y5 | 24 PAT family members | - |
Arabidopsis thaliana | Q8VYP5 | 24 PAT family members | - |
Arabidopsis thaliana | Q8VYS8 | 24 PAT family members | - |
Arabidopsis thaliana | Q93VV0 | 24 PAT family members | - |
Arabidopsis thaliana | Q94C49 | 24 PAT family members | - |
Arabidopsis thaliana | Q9C533 | 24 PAT family members | - |
Arabidopsis thaliana | Q9FLM3 | 24 PAT family members | - |
Arabidopsis thaliana | Q9LIE4 | 24 PAT family members | - |
Arabidopsis thaliana | Q9LIH7 | 24 PAT family members | - |
Arabidopsis thaliana | Q9M115 | 24 PAT family members | - |
Arabidopsis thaliana | Q9M1K5 | 24 PAT family members | - |
Arabidopsis thaliana | Q9M306 | 24 PAT family members | - |
Arabidopsis thaliana | Q9SB58 | 24 PAT family members | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | developed | Arabidopsis thaliana | - |
leaf | rosette, young, developed and bolted | Arabidopsis thaliana | - |
additional information | activity pattern of 24 PATs in the different tissues, overview | Arabidopsis thaliana | - |
pollen | - |
Arabidopsis thaliana | - |
seedling | - |
Arabidopsis thaliana | - |
stamen | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-CoA + [protein]-L-cysteine | - |
Arabidopsis thaliana | [protein]-S-palmitoyl-L-cysteine + CoA | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | identification of conserved sequence motifs within Arabidopsis PATs, e.g. the central DHHC-CRD domain, overview. The DHHC motif within the central region is always located to the vicinity of the C-terminal transmembrane domains. Protein topology of Arabidopsis PATs, overview | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
DHHC-CRD S-acyltransferase | - |
Arabidopsis thaliana |
Pat | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
evolution | the PAT enzymes of Arabidopsis thaliana belong to the DHHC-CRD-containing PAT family, PAT enzymes share a common structure mainly composed of four predicted transmembrane domains and a stretch of Asp-His-His-Cys, DHHC, within a Cys-rich domain | Arabidopsis thaliana |
additional information | expression of AtPATs can retarget Vac8p-GFP in yeast Vac8p mutant cells, overview. Vac8p-GFP is targeted to the vacuolar membrane in wild-type cells | Arabidopsis thaliana |
physiological function | protein lipid modification of cysteine residues, referred to as S-palmitoylation or S-acylation, is an important secondary and reversible modification that regulates membrane association, trafficking, and function of target proteins. This enzymatic reaction is mediated by protein S-acyl transferases, PATs | Arabidopsis thaliana |