Protein Variants | Comment | Organism |
---|---|---|
H229A | forms the C6 epimers (6R):(6S) of (2S,5S)-6-methyl-5-carboxymethylproline in the ratio of 75:25 compared to wild-type ratio of 55:45 | Pectobacterium carotovorum |
M108A | forms the C6 epimers (6R):(6S) of (2S,5S)-6-methyl-5-carboxymethylproline in the ratio of 45:55 compared to wild-type ratio of 55:45 | Pectobacterium carotovorum |
M108I | forms the C6 epimers (6R):(6S) of (2S,5S)-6-methyl-5-carboxymethylproline in the ratio of 92:8 compared to wild-type ratio of 55:45 | Pectobacterium carotovorum |
M108L | forms the C6 epimers (6R):(6S) of (2S,5S)-6-methyl-5-carboxymethylproline in the ratio of 47:53 compared to wild-type ratio of 55:45. Forms the C6 epimers (6R):(6S) of (2S,5S)-6-ethyl-5-carboxymethylproline in the ratio of 72:18 compared to wild-type ratio of 65:35. Forms the C7 epimers (7R):(7S) of 7-methyl-(2S,6S)-carboxymethylpipecolic acid in the ratio of 60:40 compared to the wild-type ration of 66:34 | Pectobacterium carotovorum |
M108V | forms the C6 epimers (6R):(6S) of (2S,5S)-6-methyl-5-carboxymethylproline in the ratio of 95:5 compared to wild-type ratio of 55:45 | Pectobacterium carotovorum |
Q111N | forms the C6 epimers (6R):(6S) of (2S,5S)-6-methyl-5-carboxymethylproline in the ratio of 70:30 compared to wild-type ratio of 55:45 | Pectobacterium carotovorum |
W79A | forms the C6 epimers (6R):(6S) of (2S,5S)-6-methyl-5-carboxymethylproline in the ratio of 16:84 compared to wild-type ratio of 55:45. Forms the C6 epimers (6R):(6S) of (2S,5S)-6-ethyl-5-carboxymethylproline in the ratio of 12:88 compared to wild-type ratio of 65:35. Forms the C7 epimers (7R):(7S) of 7-methyl-(2S,6S)-carboxymethylpipecolic acid in the ratio of 1:99 compared to the wild-type ration of 66:34 | Pectobacterium carotovorum |
W79F | forms the C6 epimers (6R):(6S) of (2S,5S)-6-methyl-5-carboxymethylproline in the ratio of 17:83 compared to wild-type ratio of 55:45. Forms the C6 epimers (6R):(6S) of (2S,5S)-6-ethyl-5-carboxymethylproline in the ratio of 32:68 compared to wild-type ratio of 65:35. Forms the C7 epimers (7R):(7S) of 7-methyl-(2S,6S)-carboxymethylpipecolic acid in the ratio of : compared to the wild-type ration of 17:83 | Pectobacterium carotovorum |
W79F/M108A | forms the C6 epimers (6R):(6S) of (2S,5S)-6-methyl-5-carboxymethylproline in the ratio of 11:89 compared to wild-type ratio of 55:45. Forms the C6 epimers (6R):(6S) of (2S,5S)-6-ethyl-5-carboxymethylproline in the ratio of 17:83 compared to wild-type ratio of 65:35. Forms the C7 epimers (7R):(7S) of 7-methyl-(2S,6S)-carboxymethylpipecolic acid in the ratio of 5:95 compared to the wild-type ration of 66:34 | Pectobacterium carotovorum |
W79F/M108V | forms the C6 epimers (6R):(6S) of (2S,5S)-6-methyl-5-carboxymethylproline in the ratio of 56:44 compared to wild-type ratio of 55:45. Forms the C6 epimers (6R):(6S) of (2S,5S)-6-ethyl-5-carboxymethylproline in the ratio of 34:66 compared to wild-type ratio of 65:35. Forms the C7 epimers (7R):(7S) of 7-methyl-(2S,6S)-carboxymethylpipecolic acid in the ratio of 24:76 compared to the wild-type ration of 66:34 | Pectobacterium carotovorum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + (S)-1-pyrroline-5-carboxylate + H2O | Pectobacterium carotovorum | - |
CoA + (2S,5S)-5-carboxymethylproline + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pectobacterium carotovorum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethylmalonyl-CoA + (2S,5S)-carboxymethylproline + CO2 | the substrate is an equilibrium mixture of L-glutamate semialdehyde, 5-hydroxy-L-proline and L-pyrroline-5-carboxylate. Some of the CarB variants catalyse the production of the two C6 epimers of (2S,5S)-6-ethyl-5-carboxymethylproline | Pectobacterium carotovorum | CoA + (2S,5S,6R)-6-ethyl-5-carboxymethylproline | wild-type enzyme produces a 65:35 mixture of (6R)- and (6S)-epimer | ? | |
ethylmalonyl-CoA + (2S,5S)-carboxymethylproline + CO2 | the substrate is an equilibrium mixture of L-glutamate semialdehyde, 5-hydroxy-L-proline and L-pyrroline-5-carboxylate. Some of the CarB variants catalyse the production of the two C6 epimers of (2S,5S)-6-ethyl-5-carboxymethylproline | Pectobacterium carotovorum | CoA + (2S,5S,6S)-6-ethyl-5-carboxymethylproline | wild-type enzyme produces a 65:35 mixture of (6R)- and (6S)-epimer | ? | |
ethylmalonyl-CoA + L-2-aminoadipate semialdehyde | activity with mutant enzymes W79F, W79F/M108A and W79F/M108V. mutant W79A: epimeric ethylmalonylCoA produces solely (2S,6S,7S)-(2S,6S)-carboxymethylpipecolic acid via reaction of (2R)-ethylmalonyl-CoA, whereas incubation of (2S)-ethylmalonylCoA and L-2-aminoadipate semialdehyde results in no detectable production of 7-ethyl-(2S,6S)-carboxymethylpipecolic acid | Pectobacterium carotovorum | CoA + (2S,6S,7R)-7-ethyl-carboxymethylpipecolic acid | - |
? | |
ethylmalonyl-CoA + L-2-aminoadipate semialdehyde | activity with mutant enzymes W79F, W79F/M108A and W79F/M108V. mutant W79A: epimeric ethylmalonylCoA produces solely (2S,6S,7S)-(2S,6S)-carboxymethylpipecolic acid via reaction of (2R)-ethylmalonyl-CoA, whereas incubation of (2S)-ethylmalonylCoA and L-2-aminoadipate semialdehyde results in no detectable production of 7-ethyl-(2S,6S)-carboxymethylpipecolic acid | Pectobacterium carotovorum | CoA + (2S,6S,7S)-7-ethyl-carboxymethylpipecolic acid | - |
? | |
malonyl-CoA + (S)-1-pyrroline-5-carboxylate + H2O | - |
Pectobacterium carotovorum | CoA + (2S,5S)-5-carboxymethylproline + CO2 | - |
? | |
malonyl-CoA + (S)-1-pyrroline-5-carboxylate + H2O | the substrate is an equilibrium mixture of L-glutamate semialdehyde, 5-hydroxy-L-proline and L-pyrroline-5-carboxylate | Pectobacterium carotovorum | CoA + (2S,5S)-5-carboxymethylproline + CO2 | - |
? | |
methylmalonyl-CoA + (2S,5S)-5-carboxymethylproline + CO2 | the substrate is an equilibrium mixture of L-glutamate semialdehyde, 5-hydroxy-L-proline and L-pyrroline-5-carboxylate | Pectobacterium carotovorum | CoA + (2S,5S,6R)-6-methyl-5-carboxymethylproline | wild-type enzyme produces a 55:45 mixture of (6R)- and (6S)-epimer | ? | |
methylmalonyl-CoA + (2S,5S)-5-carboxymethylproline + CO2 | the substrate is an equilibrium mixture of L-glutamate semialdehyde, 5-hydroxy-L-proline and L-pyrroline-5-carboxylate | Pectobacterium carotovorum | CoA + (2S,5S,6S)-6-methyl-5-carboxymethylproline | wild-type enzyme produces a 55:45 mixture of (6R)- and (6S)-epimer | ? | |
methylmalonyl-CoA + L-2-aminoadipate semialdehyde | - |
Pectobacterium carotovorum | CoA + (2S,6S,7R)-7-methyl-carboxymethylpipecolic acid | wild-type enzyme produces a 66:34 mixture of (7R)- and (7S)-epimer | ? | |
methylmalonyl-CoA + L-2-aminoadipate semialdehyde | - |
Pectobacterium carotovorum | CoA + (2S,6S,7S)-7-methyl-carboxymethylpipecolic acid | wild-type enzyme produces a 66:34 mixture of (7R)- and (7S)-epimer | ? |
Synonyms | Comment | Organism |
---|---|---|
CarB | - |
Pectobacterium carotovorum |