Cloned (Comment) | Organism |
---|---|
expression as a His-tagged fusion protein in Escherichia coli BL21 | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | - |
GDP-4-amino-4,6-dideoxy-alpha-D-mannose | pH 8.0, 37°C | Escherichia coli | |
1.8 | - |
acetyl-CoA | pH 8.0, 37°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26260 | - |
3 * 26260, His-tag PerB, SDS-PAGE | Escherichia coli |
68000 | - |
His-tag PerB, gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + GDP-4-amino-4,6-dideoxy-alpha-D-mannose | Escherichia coli | GDP-4-amino-4,6-dideoxy-alpha-D-mannose (i.e. GDP-alpha-D-perosamine) is a component of the four sugar repeated subunit of the O-antigen of Escherichia coli O157:H7. The enzyme is involved in the biosynthesis of the precursor molecule GDP-N-acetyl-D-perosamine | CoA + GDP-4-acetamido-4,6-dideoxy-alpha-D-mannose | GDP-4-acetamido-4,6-dideoxy-alpha-D-mannose i.e. GDP-N-acetyl-alpha-D-perosamine | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His-tagged fusion protein | Escherichia coli |
Storage Stability | Organism |
---|---|
4°C, 30 days, His-tag PerB, 5% loss of activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + GDP-4-amino-4,6-dideoxy-alpha-D-mannose | GDP-4-amino-4,6-dideoxy-alpha-D-mannose (i.e. GDP-alpha-D-perosamine) is a component of the four sugar repeated subunit of the O-antigen of Escherichia coli O157:H7. The enzyme is involved in the biosynthesis of the precursor molecule GDP-N-acetyl-D-perosamine | Escherichia coli | CoA + GDP-4-acetamido-4,6-dideoxy-alpha-D-mannose | GDP-4-acetamido-4,6-dideoxy-alpha-D-mannose i.e. GDP-N-acetyl-alpha-D-perosamine | ? | |
acetyl-CoA + GDP-4-amino-4,6-dideoxy-alpha-D-mannose | GDP-4-amino-4,6-dideoxy-alpha-D-mannose i.e. GDP-alpha-D-perosamine | Escherichia coli | CoA + GDP-4-acetamido-4,6-dideoxy-alpha-D-mannose | GDP-4-acetamido-4,6-dideoxy-alpha-D-mannose i.e. GDP-N-acetyl-alpha-D-perosamine | ? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 26260, His-tag PerB, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
acetyl-CoA:GDP-D-perosamine N-acetyltransferase | - |
Escherichia coli |
PerB | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
His-tag PerB, 20 min, 24% loss of activity | Escherichia coli |
75 | - |
His-tag PerB, 20 min, 90% loss of activity | Escherichia coli |
80 | - |
His-tag PerB, 10 min, complete inactivation | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
His-tag PerB protein | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | GDP-4-acetamido-4,6-dideoxy-alpha-D-mannose (i.e. GDP-N-acetyl-alpha-D-perosamine) is a component of the four sugar repeated subunit of the O-antigen of Escherichia coli O157:H7. The enzyme is involved in the biosynthesis of the precursor molecule GDP-N-acetyl-D-perosamine | Escherichia coli |