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Literature summary for 2.3.1.23 extracted from

  • Zhao, Y.; Chen, Y.Q.; Bonacci, T.M.; Bredt, D.S.; Li, S.; Bensch, W.R.; Moller, D.E.; Kowala, M.; Konrad, R.J.; Cao, G.
    Identification and characterization of a major liver lysophosphatidylcholine acyltransferase (2008), J. Biol. Chem., 283, 8258-8265.
    View publication on PubMed
Search for more literature for 2.3.1.23

Protein Variants

Protein Variants Comment Organism
additional information in a human hepatoma Huh7 cells, RNA interference-mediated knockdown of LPCAT3 results in virtually complete loss of membrane LPCAT activity, suggesting that LPCAT3 is primarily responsible for hepatic LPCAT activity Homo sapiens
additional information membranes from HEK293 cells overexpressing LPCAT3 show significantly increased LPCAT activity as assessed by thin layer chromatography analysis with substrate preference toward unsaturated fatty acids Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03565
-
 stearoyl-CoA Vmax: 996 nmol/min/mg protein Homo sapiens
0.04129
-
 palmitoyl-CoA Vmax: 1782 nmol/min/mg protein Homo sapiens
0.07268
-
 oleoyl-CoA Vmax: 4698 nmol/min/mg protein Homo sapiens
0.2014
-
 linoleoyl-CoA Vmax: 18148 nmol/min/mg protein Homo sapiens
0.7156
-
 Arachidonoyl-CoA Vmax: 6247 nmol/min/mg protein Homo sapiens
0.7219
-
 1-palmitoyl-lysophosphatidylcholine Vmax: 6364 nmol/min/mg protein Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Homo sapiens 5783
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 deduced from cDNA Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
adipose determined by RT-PCR analysis Homo sapiens
-
liver determined by RT-PCR analysis. Furthermore, peroxisome proliferator-activated receptor alpha agonists dose-dependently regulate LPCAT3 in liver in a peroxisome proliferator-activated receptor alpha-dependent fashion Homo sapiens
-
pancreas determined by RT-PCR analysis Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 among all the different substrates examined, 1-myristoyl-LPC (14:0) and 1-palmitoyl-LPC (16:0) has the most robust activity, whereas no detectable activity is observed using 1-hexanoyl (6:0) and 1-arachidoyl (20:0) LPC as substrates Homo sapiens
additional information
-
 among the saturated fatty acyl-CoAs, only lauroyl-CoA has comparable activity compared with unsaturated fatty acyl-CoAs. Oleoyl-CoA (18:1), linoleoyl-CoA (18:2), and arachidonoyl-CoA (20:4) all demonstrate robust LPCAT activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-myristoyl-lysophosphatidylcholine + palmitoyl-CoA
-
 Homo sapiens CoA + 1-myristoyl-2-palmitoylphosphatidylcholine
-
 ?
1-oleoyl-lysophosphatidylcholine + palmitoyl-CoA
-
 Homo sapiens CoA + 1-oleoyl-2-palmitoylphosphatidylcholine
-
 ?
1-palmitoyl-lysophosphatidylcholine + arachidonoyl-CoA
-
 Homo sapiens CoA + 1-palmitoyl-2-arachidonoylphosphatidylcholine
-
 ?
1-palmitoyl-lysophosphatidylcholine + lauroyl-CoA
-
 Homo sapiens CoA + 1-palmitoyl-2-lauroylphosphatidylcholine
-
 ?
1-palmitoyl-lysophosphatidylcholine + linoleoyl-CoA
-
 Homo sapiens CoA + 1-palmitoyl-2-linoleoylphosphatidylcholine
-
 ?
1-palmitoyl-lysophosphatidylcholine + n-octanoyl-CoA
-
 Homo sapiens CoA + 1-palmitoyl-2-octanoylphosphatidylcholine
-
 ?
1-palmitoyl-lysophosphatidylcholine + oleoyl-CoA
-
 Homo sapiens CoA + 2-oleoyl-1-palmitoylphosphatidylcholine
-
 ?
1-palmitoyl-lysophosphatidylcholine + palmitoyl-CoA
-
 Homo sapiens CoA + 1,2-dipalmitoylphosphatidylcholine
-
 ?
1-palmitoyl-lysophosphatidylcholine + stearoyl-CoA
-
 Homo sapiens CoA + 1-palmitoyl-2-stearoylphosphatidylcholine
-
 ?
1-stearoyl-lysophosphatidylcholine + palmitoyl-CoA
-
 Homo sapiens CoA + 1-stearoyl-2-palmitoylphosphatidylcholine
-
 ?

Synonyms

Synonyms Comment Organism
LPCAT3
-
 Homo sapiens
lysophosphatidylcholine acyltransferase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
 assay carried out at room temperature Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens