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Literature summary for 2.3.1.23 extracted from
- Substrate preferences of a lysophosphatidylcholine acyltransferase highlight its role in phospholipid remodeling (2008), Lipids, 43, 895-902.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in yeast | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | enzyme activity can be inhibited by high levels of acyl acceptors | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00045 | - |
oleoyl-CoA | Vmax: 7.4 pmol/min/microgram protein for oleoyl-CoA | Homo sapiens |  |
| 0.005 | - |
Arachidonoyl-CoA | Vmax: 12.4 pmol/min/microgram protein for arachidonoyl-CoA | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q6P1A2 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adipocyte | by retrieving LPCAT3 expression pattern from Genevestigator | Homo sapiens | - |
| heart | northern blot analysis | Homo sapiens | - |
| kidney | northern blot analysis | Homo sapiens | - |
| lung | highly expressed, detected by Northern blot analysis | Homo sapiens | - |
| pancreas | northern blot analysis | Homo sapiens | - |
| testis | northern blot analysis | Homo sapiens | - |
| thymus | highly expressed, detected by Northern blot analysis | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
LPCAT3 exhibits the highest activity with lysophosphatidylcholine. LPCAT3 exhibits substrate preferences in the order of 1-stearoyl-2-lysophosphatidylcholine > 1-arachidoyl-2-lysophosphatidylcholine > 1-O-hexadecyl-sn-glycero-3-phosphocholine > 1-palmitoyl-2-lysophosphatidylcholine | Homo sapiens |
| additional information | - |
LPCAT3 is most active in utilizing linoleoyl-CoA as acyl donor. Order range: linoleoyl-CoA > palmitoyl-CoA > arachidonoyl-CoA > oleoyl-CoA > stearoyl-CoA | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arachidonoyl-CoA + 1-arachidoyl-2-lysophosphatidylcholine | - |
Homo sapiens | CoA + 2-arachidonoyl-1-arachidoylphosphatidylcholine | - |
? | |
| arachidonoyl-CoA + 1-O-hexadecyl-sn-glycero-3-phosphocholine | LPCAT3 acylates 1-O-alkyl-sn-glycero-3-phosphocholine with arachidonyl-CoA but not with acetyl-CoA | Homo sapiens | CoA + 2-arachidonoyl-1-O-hexadecyl-sn-glycero-3-phosphocholine | - |
? | |
| arachidonoyl-CoA + 1-palmitoyl-2-lysophosphatidylcholine | - |
Homo sapiens | CoA + 2-arachidonoyl-1-palmitoylphosphatidylcholine | - |
? | |
| arachidonoyl-CoA + 1-palmitoyl-2-lysophosphatidylethanolamine | lysophosphatidylethanolamine acylation is evident. But no significant activity of acylation is detected when 16:0-lysophosphatidylglycerol is used as substrate | Homo sapiens | CoA + 2-arachidonoyl-1-palmitoylphosphatidylethanolamine | - |
? | |
| arachidonoyl-CoA + 1-stearoyl-2-lysophosphatidylcholine | - |
Homo sapiens | CoA + 2-arachidonoyl-1-stearoylphosphatidylcholine | - |
? | |
| arachidonoyl-CoA + palmitoyl-lysophosphatidylcholine | - |
Homo sapiens | CoA + 2-arachidonoyl-1-palmitoylphosphatidylcholine | - |
? | |
| linoleoyl-CoA + 1-palmitoyl-2-lysophosphatidylcholine | - |
Homo sapiens | CoA + 2-linoleoyl-1-palmitoylphosphatidylcholine | - |
? | |
| oleoyl-CoA + 1-palmitoyl-2-lysophosphatidylcholine | - |
Homo sapiens | CoA + 2-oleoyl-1-palmitoylphosphatidylcholine | - |
? | |
| oleoyl-CoA + palmitoyl-lysophosphatidylcholine | - |
Homo sapiens | CoA + 2-oleoyl-1-palmitoylphosphatidylcholine | - |
? | |
| palmitoyl-CoA + 1-palmitoyl-2-lysophosphatidylcholine | - |
Homo sapiens | CoA + 1,2-dipalmitoylphosphatidylcholine | - |
? | |
| stearoyl-CoA + 1-palmitoyl-2-lysophosphatidylcholine | - |
Homo sapiens | CoA + 2-stearoyl-1-palmitoylphosphatidylcholine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LPCAT3 | - |
Homo sapiens |
| lysophosphatidylcholine acyltransferase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
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Release 2023.1 (January 2023)
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