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Literature summary for 2.3.1.230 extracted from

  • Bera, A.K.; Atanasova, V.; Robinson, H.; Eisenstein, E.; Coleman, J.P.; Pesci, E.C.; Parsons, J.F.
    Structure of PqsD, a Pseudomonas quinolone signal biosynthetic enzyme, in complex with anthranilate (2009), Biochemistry, 48, 8644-8655.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.230

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method. Several crystal structures of the enzyme including that of the PqsD-anthranilate covalent intermediate and the inactive Cys112Ala active site mutant in complex with anthranilate Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
C112A the C112A mutant is inactive although it still reversibly binds anthraniloyl-CoA Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 2 * 36000 Pseudomonas aeruginosa
72800
-
 gel filtration, laser light scattering Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
malonyl-CoA + 2-aminobenzoyl-CoA Pseudomonas aeruginosa 2-aminobenzoyl-CoA i.e. anthraniloyl-CoA 2 CoA + 4-hydroxy-2(1H)-quinolone + CO2 i.e. 2,4-dihydroxyquinoline ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa P20582
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-CoA + 2-aminobenzoyl-CoA 2-aminobenzoyl-CoA i.e. anthraniloyl-CoA Pseudomonas aeruginosa 2 CoA + 4-hydroxy-2(1H)-quinolone + CO2 i.e. 2,4-dihydroxyquinoline ?

Subunits

Subunits Comment Organism
homodimer 2 * 36000 Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
PqsD
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Pseudomonas aeruginosa

General Information

General Information Comment Organism
physiological function PqsD is a quinolone signal biosynthetic enzyme Pseudomonas aeruginosa