Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-(4-bromo-3-diethylsulfamoylbenzoylamino)benzoate | - |
Pseudomonas aeruginosa | |
2-[(2-phenoxybiphenyl-4-carbonyl)amino]benzoate | - |
Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0487 | - |
malonyl-CoA | pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
0.599 | - |
2-aminobenzoyl-CoA | pH and temperature not specified in the publication | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38440 | - |
HPLC mass spectrometry | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + 2-aminobenzoyl-CoA | Pseudomonas aeruginosa | the enzyme is involved in the biosynthesis of the signal molecules 2-heptyl-3-hydroxy-4(1H)-quinolone and 4-hydroxy-2(1H)-quinolone. These compounds are part of the quorum-sensing systems that is based on the production and release of small signaling molecules, called autoinducers, that increase in concentration as a function of cell density and activate corresponding transcriptional regulators after a threshold concentration has been reached | 2 CoA + 4-hydroxy-2(1H)-quinolone + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-oxodecanoate + 2-aminobenzoyl-CoA | - |
Pseudomonas aeruginosa | CoA + 2-heptyl-4(1H)-quinolone + CO2 + H2O | - |
? | |
3-oxodecanoyl-CoA + 2-aminobenzoyl-CoA | high concentrations and a longer incubation time is required in comparison to the free 3-oxodecanoic acid | Pseudomonas aeruginosa | 2 CoA + 2-heptyl-3-hydroxy-4(1H)-quinolone + CO2 | - |
? | |
3-oxodecanoyl-N-acetylcysteamine + 2-aminobenzoyl-CoA | high concentrations and a longer incubation time is required in comparison to the free 3-oxodecanoic acid | Pseudomonas aeruginosa | N-acetylcysteamine + 2-heptyl-3-hydroxy-4(1H)-quinolone + CO2 | - |
? | |
3-oxohexanoate + 2-aminobenzoyl-CoA | - |
Pseudomonas aeruginosa | CoA + 2-propyl-4(1H)-quinolone + CO2 + H2O | - |
? | |
3-oxopentanoate + 2-aminobenzoyl-CoA | - |
Pseudomonas aeruginosa | CoA + 2-ethyl-4(1H)-quinolone + CO2 + H2O | - |
? | |
malonyl-CoA + 2-aminobenzoyl-CoA | the enzyme is involved in the biosynthesis of the signal molecules 2-heptyl-3-hydroxy-4(1H)-quinolone and 4-hydroxy-2(1H)-quinolone. These compounds are part of the quorum-sensing systems that is based on the production and release of small signaling molecules, called autoinducers, that increase in concentration as a function of cell density and activate corresponding transcriptional regulators after a threshold concentration has been reached | Pseudomonas aeruginosa | 2 CoA + 4-hydroxy-2(1H)-quinolone + CO2 | - |
? | |
malonyl-CoA + 2-aminobenzoyl-CoA | catalyzes the decarboxylative Claisen condensation with malonyl-CoA. | Pseudomonas aeruginosa | 2 CoA + 4-hydroxy-2(1H)-quinolone + CO2 | - |
? |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.035 | - |
pH and temperature not specified in the publication | Pseudomonas aeruginosa | 2-[(2-phenoxybiphenyl-4-carbonyl)amino]benzoate | |
0.065 | - |
pH and temperature not specified in the publication | Pseudomonas aeruginosa | 2-(4-bromo-3-diethylsulfamoylbenzoylamino)benzoate |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
2-aminobenzoyl-CoA | pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
69 | - |
malonyl-CoA | pH and temperature not specified in the publication | Pseudomonas aeruginosa |