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Literature summary for 2.3.1.24 extracted from
- Partial purification and characterization of sphingosine N-acyltransferase (ceramide synthase) from bovine liver mitochondrion-rich fraction (1998), Lipids, 33, 601-605.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.141 | - |
palmitoyl-CoA | - |
Bos taurus |  |
| 0.144 | - |
sphinganine | - |
Bos taurus | |
| 0.146 | - |
stearoyl-CoA | - |
Bos taurus | |
| 0.171 | - |
sphingosine | - |
Bos taurus | |
| 0.18 | - |
oleoyl-CoA | - |
Bos taurus | |
| 0.299 | - |
behenoyl-CoA | - |
Bos taurus | |
| 0.358 | - |
lauroyl-CoA | - |
Bos taurus | |
| 0.522 | - |
hexanoyl-CoA | - |
Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
x * 62000 + x * 72000, SDS-PAGE | Bos taurus |
| 72000 | - |
x * 62000 + x * 72000, SDS-PAGE | Bos taurus |
| 240000 | 260000 | gel filtration | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| n-octyl beta-D-thioglucoside, DE-32, sphingosine-celite, Sepharose CL-6B | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Bos taurus | - |
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0148 | - |
- |
Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| an acyl-CoA + sphinganine | - |
Bos taurus | CoA + an N-acylsphinganine | - |
? | |
| an acyl-CoA + sphingosine | - |
Bos taurus | CoA + an N-acylsphingosine | - |
? | |
| behenoyl-CoA + sphingosine | - |
Bos taurus | CoA + N-behenoylsphingosine | - |
? | |
| hexanoyl-CoA + sphingosine | - |
Bos taurus | CoA + N-hexanoylsphingosine | - |
? | |
| lauroyl-CoA + sphingosine | - |
Bos taurus | CoA + N-lauroylsphingosine | - |
? | |
| oleoyl-CoA + sphingosine | - |
Bos taurus | CoA + N-oleoylsphingosine | - |
? | |
| palmitoyl-CoA + sphingosine | - |
Bos taurus | CoA + N-palmitoylsphingosine | - |
? | |
| stearoyl-CoA + sphingosine | - |
Bos taurus | CoA + N-stearoylsphingosine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 62000 + x * 72000, SDS-PAGE | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | more than 70% of maximal activity between pH 7.0 and pH 8.0 | Bos taurus |
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Release 2023.1 (January 2023)
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