Cloned (Comment) | Organism |
---|---|
- |
Amycolatopsis orientalis |
Protein Variants | Comment | Organism |
---|---|---|
C160A/C190A | formation of (3,5-dihydroxyphenylacetyl)-CoA is barely detectable | Amycolatopsis orientalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41593 | - |
2 * 41593, electrospray mass spectrometry | Amycolatopsis orientalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Amycolatopsis orientalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Amycolatopsis orientalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 malonyl-CoA | diketyl-CoA and triketyl-CoA are confirmed to be the intermediates in the enzyme catalyzed synthesis of (3,5-dihydroxyphenylacetyl)-CoA. Polyketone intermediates alternate between the Cys160 residue and CoA through transthioesterification reactions, in which the chain is elongated by enolate attack on the thioester carbon of the enzyme-bound (poly)ketide intermediate by decarboxylation in malonyl-CoA or polyketide-CoA. Polyketone cyclization likely arises on CoA but not on the enzyme. Experimental data suggest that cyclization of linear tetraketidyl-CoA occurs in concert with or before decarboxylation of the terminal carboxyl group | Amycolatopsis orientalis | (3,5-dihydroxyphenylacetyl)-CoA + 3 CoA + 4 CO2 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 41593, electrospray mass spectrometry | Amycolatopsis orientalis |
Synonyms | Comment | Organism |
---|---|---|
dpgA | - |
Amycolatopsis orientalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Amycolatopsis orientalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Amycolatopsis orientalis |