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Literature summary for 2.3.1.247 extracted from

  • Barker, H.A.; Kahn, J.M.; Chew, S.
    Enzymes involved in 3,5-diaminohexanoate degradation by Brevibacterium sp. (1980), J. Bacteriol., 143, 1165-1170.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.247

Inhibitors

Inhibitors Comment Organism Structure
additional information no effect on enzyme activity by 0.5 mM DTNB Brevibacterium sp.
phosphate weak inhibition Brevibacterium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no effect on enzyme activity by Co2+ and Mg2+ at 0.1 mM Brevibacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
123000
-
 native enzyme, gel filtration Brevibacterium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(5S)-5-amino-3-oxohexanoate + acetyl-CoA Brevibacterium sp.
-
 L-3-aminobutanoyl-CoA + acetoacetate
-
 r
(5S)-5-amino-3-oxohexanoate + acetyl-CoA Brevibacterium sp. L5
-
 L-3-aminobutanoyl-CoA + acetoacetate
-
 r

Organism

Organism UniProt Comment Textmining
Brevibacterium sp.
-
-
-
Brevibacterium sp. L5
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native soluble enzyme 78fold by ammonium sulfate fractionation and anion exchange chromatography, followed by ultrafiltration and adsorption chromatography Brevibacterium sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:DL-erythro-3,5-diaminohexanoate-grown cell
-
 Brevibacterium sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.105
-
 crude enzyme extract, pH 7.0, 25°C Brevibacterium sp.
12.5
-
 purified enzyme, pH 7.0, 25°C Brevibacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5S)-5-amino-3-oxohexanoate + acetyl-CoA
-
 Brevibacterium sp. L-3-aminobutanoyl-CoA + acetoacetate
-
 r
(5S)-5-amino-3-oxohexanoate + acetyl-CoA preferred substrate, the substrate is cleaved between C-2 and C-3 to form C-2 and C-4 moieties Brevibacterium sp. L-3-aminobutanoyl-CoA + acetoacetate DL-3-aminobutyryl-CoA and acetoacetate are used in the reverse reaction assay r
(5S)-5-amino-3-oxohexanoate + acetyl-CoA
-
 Brevibacterium sp. L5 L-3-aminobutanoyl-CoA + acetoacetate
-
 r
(5S)-5-amino-3-oxohexanoate + acetyl-CoA preferred substrate, the substrate is cleaved between C-2 and C-3 to form C-2 and C-4 moieties Brevibacterium sp. L5 L-3-aminobutanoyl-CoA + acetoacetate DL-3-aminobutyryl-CoA and acetoacetate are used in the reverse reaction assay r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Brevibacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 forward reaction assay at Brevibacterium sp.
8
-
 reverse reaction Brevibacterium sp.

General Information

General Information Comment Organism
metabolism C-1 and C-2 of 3-oxo-5-aminohexanoate are converted mainly to acetoacetate and acetate, whereas C-3 to C-6 are converted mainly to 3-hydroxybutyrate or its coenzyme A thiolester. 3-Aminobutyryl-CoA is then deaminated to form crotonyl-CoA. The enzymes observed in extracts of Brevibacterium sp. can account for the conversion of 3,5-diaminohexanoate to acetyl-CoA, overview Brevibacterium sp.