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Literature summary for 2.3.1.250 extracted from

  • Miranda, M.; Galli, L.M.; Enriquez, M.; Szabo, L.A.; Gao, X.; Hannoush, R.N.; Burrus, L.W.
    Identification of the WNT1 residues required for palmitoylation by Porcupine (2014), FEBS Lett., 588, 4815-4824.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.250

Activating Compound

Activating Compound Comment Organism Structure
additional information substrate glycosylation promotes, but is not absolutely required for Wnt1 palmitoylation Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound Homo sapiens 16020
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine Homo sapiens
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 CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
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 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9H237
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-

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293T cell
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 Homo sapiens
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(9Z)-hexadec-9-enoyl-CoA + [Wnt1]-L-serine chick WNT1 protein, the enzyme is an O-acyl transferase for WNT1 protein, WNT1 residues 214-234 are sufficient for PORCN-dependent palmitoylation of Ser224. Substitution of Ser224 with Thr, but not Cys, is tolerated in palmitoylation and biological assays. Transiently transfection of HEK-293T cells with WNT1. WNT1 mutant S224T is accepted as substrate, while mutants S224A and S224C are inactive Homo sapiens CoA + [Wnt1]-O-(9Z)-hexadec-9-enoyl-L-serine
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 ?
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine
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 Homo sapiens CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
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 ?
additional information generation of a fusion protein that is palmitoylated in a PORCN-dependent manner, overview Homo sapiens ?
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 ?

Synonyms

Synonyms Comment Organism
PORCN
-
 Homo sapiens

General Information

General Information Comment Organism
evolution the enzyme is a member of the membrane-bound O-acyl transferase (MBOAT) superfamily Homo sapiens
physiological function the enzyme is enzyme responsible for palmitoylating Wnt proteins, importance of enzyme catalyzed palmitoylation for WNT1 activity and Wnt signaling Homo sapiens