Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetone | about 40% inhibition at a final concentration of 1% v/v | Saccharomyces cerevisiae | |
butanol | about 40% inhibition at a final concentration of 1% v/v | Saccharomyces cerevisiae | |
ethanol | about 40% inhibition at a final concentration of 1% v/v | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
cholesterol | with oleoyl-CoA | Saccharomyces cerevisiae | |
0.069 | - |
oleoyl-CoA | - |
Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Saccharomyces cerevisiae | - |
- |
spheroplast | - |
Saccharomyces cerevisiae | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + cholesterol | 16% of the activity with oleoyl-CoA | Saccharomyces cerevisiae | CoA + cholesteryl acetate | - |
? | |
acyl-CoA + cholesterol | acyl-CoA derivatives can be replaced by ATP, Mg2+, CoA and fatty acids, in-situ production of acyl-CoA | Saccharomyces cerevisiae | CoA + cholesterol ester | - |
? | |
lauroyl-CoA + cholesterol | 62% of the activity with oleoyl-CoA | Saccharomyces cerevisiae | CoA + cholesteryl laurate | - |
? | |
additional information | the enzyme has a broad substrate specificity for sterols and acyl-CoAs | Saccharomyces cerevisiae | ? | - |
? | |
myristoyl-CoA + cholesterol | 37% of the activity with oleoyl-CoA | Saccharomyces cerevisiae | CoA + cholesteryl myristate | - |
? | |
oleoyl-CoA + cholesterol | highest activity | Saccharomyces cerevisiae | CoA + cholesteryl oleate | - |
? | |
oleoyl-CoA + episterol | 62% of the activity with cholesterol | Saccharomyces cerevisiae | CoA + episteryl oleate | - |
? | |
oleoyl-CoA + ergosterol | 147% of the activity with cholesterol | Saccharomyces cerevisiae | CoA + ergosteryl oleate | - |
? | |
oleoyl-CoA + fecosterol | 48% of the activity with cholesterol | Saccharomyces cerevisiae | CoA + fecosteryl oleate | - |
? | |
oleoyl-CoA + lanosterol | 65% of the activity with cholesterol | Saccharomyces cerevisiae | CoA + lanosteryl oleate | - |
? | |
oleoyl-CoA + zymosterol | 93% of the activity with cholesterol | Saccharomyces cerevisiae | CoA + zymosteryl oleate | - |
? | |
palmitoleoyl-CoA + cholesterol | 91% of the activity with oleoyl-CoA | Saccharomyces cerevisiae | CoA + cholesteryl palmitoleate | - |
? | |
palmitoyl-CoA + cholesterol | 47% of the activity with oleoyl-CoA | Saccharomyces cerevisiae | CoA + cholesteryl palmitate | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
CoA | required by the in-situ acyl-CoA production | Saccharomyces cerevisiae |