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Literature summary for 2.3.1.26 extracted from
- Cholesterol acyltransferase (1985), Methods Enzymol., 111, 286-293.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| albumin | - |
Homo sapiens | |
| albumin | required in the assay medium to prevent the high concentration of oleoly-CoA from inhibiting the enzyme presumably by disrupting the microsomal membrane | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (Z)-17(20)-dehydrocholesterol | inhibits esterification of endogenous cholesterol | Rattus norvegicus |  |
| 20-Methylcholesterol | inhibits esterification of endogenous cholesterol | Rattus norvegicus | |
| 5alpha-dihydroprogesterone | inhibits esterification of endogenous cholesterol | Homo sapiens | |
| acetone | - |
Rattus norvegicus | |
| acetone | about 40% inhibition at a final concentration of 1% v/v | Saccharomyces cerevisiae | |
| androsterone | inhibits esterification of endogenous cholesterol | Rattus norvegicus | |
| butanol | - |
Rattus norvegicus | |
| butanol | about 40% inhibition at a final concentration of 1% v/v | Saccharomyces cerevisiae | |
| Cycloartenol | inhibits esterification of exogenous cholesterol | Rattus norvegicus | |
| deoxycorticosterone | inhibits esterification of endogenous cholesterol | Homo sapiens | |
| deoxycorticosterone | - |
Rattus norvegicus | |
| Detergents | - |
Homo sapiens | |
| Detergents | above their critical micelle concentration, e.g. cholate, deoxycholate, cetyltriethylammonium bromide, Triton X-100, Tween 20, Tween 80, Nonidet P40 | Rattus norvegicus | |
| Detergents | - |
Sus scrofa | |
| Digitonin | - |
Rattus norvegicus | |
| ergosterol | inhibits esterification of exogenous cholesterol | Rattus norvegicus | |
| ethanol | - |
Rattus norvegicus | |
| ethanol | about 40% inhibition at a final concentration of 1% v/v | Saccharomyces cerevisiae | |
| Filipin | - |
Rattus norvegicus | |
| lanosterol | inhibits esterification of exogenous cholesterol | Helicoverpa zea | |
| lanosterol | inhibits esterification of exogenous cholesterol | Rattus norvegicus | |
| oleoyl-CoA | inhibits at high concentrations, the inhibition is prevented by serum albumin | Rattus norvegicus | |
| organic solvents | inhibit by perturbing the membrane structure | Rattus norvegicus | |
| Pregn-5-en-3beta-ol | inhibits esterification of endogenous cholesterol | Rattus norvegicus | |
| pregnenolone | inhibits esterification of endogenous cholesterol | Homo sapiens | |
| progesterone | inhibits esterification of endogenous cholesterol reversibly | Homo sapiens | |
| progesterone | inhibits esterification of endogenous cholesterol reversibly | Rattus norvegicus | |
| SC-31769 | 20-oxa analog of 7-ketosterol | Homo sapiens | |
| SC-31769 | 20-oxa analog of 7-ketosterol | Rattus norvegicus | |
| sitosterol | inhibits esterification of exogenous cholesterol | Rattus norvegicus | |
| stanolone | inhibits esterification of endogenous cholesterol | Rattus norvegicus | |
| stigmasterol | inhibits esterification of exogenous cholesterol | Rattus norvegicus | |
| testosterone | inhibits esterification of endogenous cholesterol | Homo sapiens | |
| testosterone | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Homo sapiens | |
| additional information | - |
additional information | use of kinetic parameters such as Km is of limited value due to the physical properties of oleoyl-CoA in solution and the addition of serum albumin to the assay | Rattus norvegicus | |
| 0.055 | - |
oleoyl-CoA | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
| endoplasmic reticulum | - |
Rattus norvegicus | 5783 | - |
| microsome | - |
Homo sapiens | - |
- |
| microsome | - |
Rattus norvegicus | - |
- |
| microsome | - |
Sus scrofa | - |
- |
| microsome | - |
Saccharomyces cerevisiae | - |
- |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| additional information | the use of organic solvents or strong detergents for the suspension of cholesterol must be avoided because of their affect on the enzyme | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helicoverpa zea | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
| Saccharomyces cerevisiae | - |
- |
- |
| Sus scrofa | - |
- |
- |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| the enzyme must be reconstituted into an artificial membrane for optimal activity | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
| liver | - |
Rattus norvegicus | - |
| liver | - |
Sus scrofa | - |
| peritoneal macrophage | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Saccharomyces cerevisiae |
| additional information | - |
assay method | Homo sapiens |
| additional information | - |
assay method | Rattus norvegicus |
| additional information | - |
assay method | Sus scrofa |
| additional information | - |
assay method | Helicoverpa zea |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, stable for several weeks | Rattus norvegicus |
| -80°C, stable for several weeks | Saccharomyces cerevisiae |
| -80°C, stable for several weeks | Helicoverpa zea |
| ovary, labile to freezing | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + cholesterol | - |
Homo sapiens | CoA + cholesterol ester | - |
? | |
| acyl-CoA + cholesterol | - |
Rattus norvegicus | CoA + cholesterol ester | - |
? | |
| acyl-CoA + cholesterol | - |
Sus scrofa | CoA + cholesterol ester | - |
? | |
| acyl-CoA + cholesterol | - |
Saccharomyces cerevisiae | CoA + cholesterol ester | - |
? | |
| acyl-CoA + cholesterol | - |
Helicoverpa zea | CoA + cholesterol ester | - |
? | |
| linoleoyl-CoA + cholesterol | - |
Rattus norvegicus | CoA + cholesteryl linoleate | - |
? | |
| additional information | - |
Homo sapiens | ? | - |
? | |
| additional information | - |
Saccharomyces cerevisiae | ? | - |
? | |
| additional information | a part of the substrate selectivity may be due to the presence of acyl-CoA hydrolase in the microsomal preparations. For this enzyme, the preferred substrate is palmitoyl-CoA which is hydrolysed about 8times faster than oleoyl-CoA | Rattus norvegicus | ? | - |
? | |
| additional information | investigations into the sterol specificity of the enzyme | Rattus norvegicus | ? | - |
? | |
| oleoyl-CoA + cholesterol | - |
Homo sapiens | CoA + cholesteryl oleate | - |
? | |
| oleoyl-CoA + cholesterol | - |
Sus scrofa | CoA + cholesteryl oleate | - |
? | |
| oleoyl-CoA + cholesterol | highest activity | Rattus norvegicus | CoA + cholesteryl oleate | - |
? | |
| oleoyl-CoA + cholesterol | highest activity | Saccharomyces cerevisiae | CoA + cholesteryl oleate | - |
? | |
| oleoyl-CoA + ergosterol | - |
Homo sapiens | CoA + ergosteryl oleate | - |
? | |
| oleoyl-CoA + ergosterol | - |
Saccharomyces cerevisiae | CoA + ergosteryl oleate | - |
? | |
| palmitoyl-CoA + cholesterol | - |
Rattus norvegicus | CoA + cholesteryl palmitate | - |
? | |
| palmitoyl-CoA + cholesterol | - |
Saccharomyces cerevisiae | CoA + cholesteryl palmitate | - |
? | |
| stearoyl-CoA + cholesterol | - |
Rattus norvegicus | CoA + cholesteryl stearate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
| 37 | - |
assay at | Rattus norvegicus |
| 37 | - |
assay at | Sus scrofa |
| 37 | - |
assay at | Helicoverpa zea |
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