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Literature summary for 2.3.1.26 extracted from
- Differential expression of ACAT1 and ACAT2 among cells within liver, intestine, kidney, and adrenal of nonhuman primates (2000), J. Lipid Res., 41, 1991-2001.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in AC29 cells | Platyrrhini |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | ACAT2: rough | Platyrrhini | 5783 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
immunoblotting, ACAT2 enzyme | Platyrrhini |
| 53000 | - |
immunoblotting, ACAT1 enzyme | Platyrrhini |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| long-chain fatty acyl-CoA + cholesterol | Platyrrhini | - |
CoA + cholesteryl long-chain fatty acyl ester | - |
? |  |
| additional information | Platyrrhini | two closely related enzymes identified that catalyse the esterification of cholesterol using acyl-CoA substrates, namely ACAT1 and ACAT2, the orientation of ACAT2 in the endoplasmic reticulum membrane, in addition to its expression only in liver and intestine, suggests that this enzyme may have as a primary function, the secretion of cholesteryl esters into apolipoprotein B-containing lipoproteins | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Platyrrhini | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adrenal gland | ACAT1: strongly present in the cells of the cortex | Platyrrhini | - |
| glomerulus | ACAT1, distal tubules and podocytes | Platyrrhini | - |
| goblet cell | ACAT1 | Platyrrhini | - |
| hepatocyte | two different forms of the enzyme are present, ACAT1 and ACAT2 | Platyrrhini | - |
| intestine | ACAT2: strongly present in the apical third of the mucosal cells, ACAT1: strong signal in goblet cells, Paneth cells and villus macrophages | Platyrrhini | - |
| jejunum | - |
Platyrrhini | - |
| kidney | - |
Platyrrhini | - |
| Kupffer cell | ACAT1 present | Platyrrhini | - |
| liver | - |
Platyrrhini | - |
| macrophage | ACAT1, villus | Platyrrhini | - |
| paneth cell | ACAT1 | Platyrrhini | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00015 | - |
kidney microsomes | Platyrrhini |
| 0.00087 | - |
small intestine microsomes | Platyrrhini |
| 0.00135 | - |
liver microsomes | Platyrrhini |
| 0.0037 | - |
adrenal microsomes | Platyrrhini |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| long-chain fatty acyl-CoA + cholesterol | - |
Platyrrhini | CoA + cholesteryl long-chain fatty acyl ester | - |
? | |
| additional information | two closely related enzymes identified that catalyse the esterification of cholesterol using acyl-CoA substrates, namely ACAT1 and ACAT2, the orientation of ACAT2 in the endoplasmic reticulum membrane, in addition to its expression only in liver and intestine, suggests that this enzyme may have as a primary function, the secretion of cholesteryl esters into apolipoprotein B-containing lipoproteins | Platyrrhini | ? | - |
? | |
| oleoyl-CoA + cholesterol | - |
Platyrrhini | CoA + cholesteryl oleate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Platyrrhini |
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