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Literature summary for 2.3.1.26 extracted from
- Human acyl-CoA: cholesterol acyltransferase inhibitory activities of aliphatic acid amides from Zanthoxylum piperitum DC (2007), Biol. Pharm. Bull., 30, 205-207.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the baculovirus system is used | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-[(2E,6Z,8E,10E)-dodeca-2,6,8,10-tetraenoylamino]-1,1-dimethylethyl acetate | IC50: 0.0281 mM; IC50: 0.0875 mM | Homo sapiens |  |
| beta-sanshool | extracted from Zanthoxylum piperitum DC; IC50: 0.012 mM; IC50: 0.039 mM | Homo sapiens | |
| beta-sanshool acetate | semi-synthetic compound | Homo sapiens | |
| gamma-sanshool | extracted from Zanthoxylum piperitum DC; IC50: 0.0797 mM; IC50: 0.0826 mM | Homo sapiens | |
| hydroxy-beta-sanshool | low inhibition; low inhibition | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + cholesterol | Homo sapiens | - |
CoA + cholesterol ester | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Homo sapiens | O75908 | - |
- |
| Homo sapiens | P35610 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| microsomal fractions of Hi5 cells containing baculovirally expressed hACAT-1 are prepared and used as the source for the enzyme | Homo sapiens |
| microsomal fractions of Hi5 cells containing baculovirally expressed hACAT-2 are prepared and used as the source for the enzyme | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + cholesterol | - |
Homo sapiens | CoA + cholesterol ester | - |
? | |
| oleoyl-CoA + cholesterol | - |
Homo sapiens | CoA + cholesteryl oleate | - |
? | |
| oleoyl-CoA + cholesterol | ACAT activity assay | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ACAT-1 | - |
Homo sapiens |
| ACAT-2 | - |
Homo sapiens |
| acyl-CoA: cholesterol acyltransferase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
activity assay | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
activity assay | Homo sapiens |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
- |
Homo sapiens | beta-sanshool | |
| 0.012 | - |
IC50: 0.012 mM | Homo sapiens | beta-sanshool | |
| 0.0281 | - |
- |
Homo sapiens | beta-sanshool acetate | |
| 0.0281 | - |
IC50: 0.0281 mM | Homo sapiens | 2-[(2E,6Z,8E,10E)-dodeca-2,6,8,10-tetraenoylamino]-1,1-dimethylethyl acetate | |
| 0.039 | - |
- |
Homo sapiens | beta-sanshool | |
| 0.039 | - |
IC50: 0.039 mM | Homo sapiens | beta-sanshool | |
| 0.0797 | - |
- |
Homo sapiens | gamma-sanshool | |
| 0.0797 | - |
IC50: 0.0797 mM | Homo sapiens | gamma-sanshool | |
| 0.0826 | - |
- |
Homo sapiens | gamma-sanshool | |
| 0.0826 | - |
IC50: 0.0826 mM | Homo sapiens | gamma-sanshool | |
| 0.0875 | - |
- |
Homo sapiens | beta-sanshool acetate | |
| 0.0875 | - |
IC50: 0.0875 mM | Homo sapiens | 2-[(2E,6Z,8E,10E)-dodeca-2,6,8,10-tetraenoylamino]-1,1-dimethylethyl acetate | |
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