Cloned (Comment) | Organism |
---|---|
into the PCDNA3 vector | Chlorocebus aethiops |
into the PCDNA3 vector | Chlorocebus sabaeus |
Protein Variants | Comment | Organism |
---|---|---|
A1D400N | mutant, evaluation of conserved aspartic acid residues | Chlorocebus aethiops |
A1D406A | mutant, evaluation of conserved aspartic acid residues | Chlorocebus aethiops |
A1H386N | mutant, identification of essential histidines required for activity | Chlorocebus aethiops |
A1H425A | mutant, identification of essential histidines required for activity | Chlorocebus aethiops |
A1H460A | mutant, identification of essential histidines required for activity | Chlorocebus aethiops |
A1S128A | mutant, identification of the role of conserved serine residues in determination of the activity | Chlorocebus aethiops |
A1S194A | mutant, identification of the role of conserved serine residues in determination of the activity | Chlorocebus aethiops |
A1S269A | mutant, identification of the role of conserved serine residues in determination of the activity | Chlorocebus aethiops |
A1S410A | mutant, identification of the role of conserved serine residues in determination of the activity | Chlorocebus aethiops |
A1S412A | mutant, identification of the role of conserved serine residues in determination of the activity | Chlorocebus aethiops |
A1S414L | mutant, identification of the role of conserved serine residues in determination of the activity | Chlorocebus aethiops |
A1S456A | mutant, identification of the role of conserved serine residues in determination of the activity | Chlorocebus aethiops |
A1Y142F | mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity | Chlorocebus aethiops |
A1Y308F | mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity | Chlorocebus aethiops |
A1Y312F | mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity | Chlorocebus aethiops |
A1Y322F | mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity | Chlorocebus aethiops |
A1Y404F | mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity | Chlorocebus aethiops |
A1Y433F | mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity | Chlorocebus aethiops |
A1Y518F | mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity | Chlorocebus aethiops |
A2D378E | mutant, evaluation of conserved aspartic acid residues | Chlorocebus sabaeus |
A2D384A | mutant, evaluation of conserved aspartic acid residues | Chlorocebus sabaeus |
A2H364N | mutant, identification of essential histidines required for activity | Chlorocebus sabaeus |
A2H403A | mutant, identification of essential histidines required for activity | Chlorocebus sabaeus |
A2H438A | mutant, identification of essential histidines required for activity | Chlorocebus sabaeus |
A2S109A | mutant, identification of the role of conserved serine residues in determination of the activity | Chlorocebus sabaeus |
A2S176A | mutant, identification of the role of conserved serine residues in determination of the activity | Chlorocebus sabaeus |
A2S249A | mutant, identification of the role of conserved serine residues in determination of the activity | Chlorocebus sabaeus |
A2S388A | mutant, identification of the role of conserved serine residues in determination of the activity | Chlorocebus sabaeus |
A2S390A | mutant, identification of the role of conserved serine residues in determination of the activity | Chlorocebus sabaeus |
A2S392A | mutant, identification of the role of conserved serine residues in determination of the activity | Chlorocebus sabaeus |
A2S434A | mutant, identification of the role of conserved serine residues in determination of the activity | Chlorocebus sabaeus |
A2Y124F | mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity | Chlorocebus sabaeus |
A2Y286F | mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity | Chlorocebus sabaeus |
A2Y290F | mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity | Chlorocebus sabaeus |
A2Y300F | mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity | Chlorocebus sabaeus |
A2Y382F | mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity | Chlorocebus sabaeus |
A2Y411F | mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity | Chlorocebus sabaeus |
A2Y496F | mutant, evaluation of the role of conserved tyrosine residues as a requirement for the activity | Chlorocebus sabaeus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + cholesterol | Chlorocebus aethiops | - |
CoA + cholesterol ester | - |
? | |
acyl-CoA + cholesterol | Chlorocebus sabaeus | - |
CoA + cholesterol ester | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlorocebus aethiops | - |
African green monkey | - |
Chlorocebus sabaeus | - |
African green monkey | - |
Purification (Comment) | Organism |
---|---|
microsomes from AC29 cells are prepared | Chlorocebus aethiops |
microsomes from AC29 cells are prepared | Chlorocebus sabaeus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + cholesterol | - |
Chlorocebus aethiops | CoA + cholesterol ester | - |
? | |
acyl-CoA + cholesterol | - |
Chlorocebus sabaeus | CoA + cholesterol ester | - |
? | |
oleoyl-CoA + cholesterol | activity assay | Chlorocebus sabaeus | CoA + cholesteryl oleate | - |
? | |
oleoyl-CoA + cholesterol | activity assay | Chlorocebus aethiops | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ACAT1 | - |
Chlorocebus aethiops |
ACAT2 | - |
Chlorocebus sabaeus |
acyl-CoA:cholesterol acyltransferase 1 | - |
Chlorocebus aethiops |
acyl-CoA:cholesterol acyltransferase 2 | - |
Chlorocebus sabaeus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
microsomal ACAT assay | Chlorocebus aethiops |
37 | - |
microsomal ACAT assay | Chlorocebus sabaeus |