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Literature summary for 2.3.1.28 extracted from
- Characterization of the chloramphenicol acetyltransferase variants encoded by the plasmids pSCS6 and pSCS7 from Staphylococcus aureus (1992), J. Gen. Microbiol., 138, 275-281.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0025 | - |
chloramphenicol | enzyme from plasmid pSCS6 | Staphylococcus aureus |  |
| 0.0027 | - |
chloramphenicol | enzyme from plasmid pSCS7 | Staphylococcus aureus | |
| 0.056 | - |
acetyl-CoA | enzyme from plasmid pSCS7 | Staphylococcus aureus | |
| 0.059 | - |
acetyl-CoA | enzyme from plasmid pSCS6 | Staphylococcus aureus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| HgCl2 | at 0.1 M and 0.3 mM, no influence on the activity of the pSCS6-encoded enzyme. The activity of the pSCS7-encoded enzyme decreases to 10-15%. At 1.0 mM HgCl2, the pSCS6-encoded enzyme retains 50% of ist original activity, complete inactivation of the enzyme from pSCS7-encoded enzyme | Staphylococcus aureus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
3 * 23000, SDS-PAGE | Staphylococcus aureus |
| 70000 | - |
gel filtration | Staphylococcus aureus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + chloramphenicol | Staphylococcus aureus | inducible enzyme | CoA + chloramphenicol 3-acetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | - |
encoded by plasmids pSCS6 or pSCS7 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Staphylococcus aureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + chloramphenicol | - |
Staphylococcus aureus | CoA + chloramphenicol 3-acetate | - |
? | |
| acetyl-CoA + chloramphenicol | inducible enzyme | Staphylococcus aureus | CoA + chloramphenicol 3-acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 23000, SDS-PAGE | Staphylococcus aureus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
15 min, 40% loss of activity of enzyme variant from plasmid pSCS6, 45% loss of activity of enzyme variant pSCS7 | Staphylococcus aureus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Staphylococcus aureus |
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