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Literature summary for 2.3.1.3 extracted from

  • Qin, Z.; Xiao, Y.; Yang, X.; Mesters, J.R.; Yang, S.; Jiang, Z.
    A unique GCN5-related glucosamine N-acetyltransferase region exist in the fungal multi-domain glycoside hydrolase family 3 beta-N-acetylglucosaminidase (2015), Sci. Rep., 5, 18292.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-Gold (DE3) cells Rhizomucor miehei

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 1.3-1.6 M (NH4)2SO4, 0.1 M Bis-Tris pH (5.5–6.5) at 20°C Rhizomucor miehei

Protein Variants

Protein Variants Comment Organism
H842A inactive Rhizomucor miehei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
230000
-
 gel filtration Rhizomucor miehei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + D-glucosamine Rhizomucor miehei
-
 CoA + N-acetyl-D-glucosamine
-
 ?

Organism

Organism UniProt Comment Textmining
Rhizomucor miehei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex 200 gel filtration Rhizomucor miehei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + chitobiose 77.5% activity compared to D-glucosamine Rhizomucor miehei CoA + N,N'-diacetylchitobiose + N-acetyl-D-glucosamine
-
 ?
acetyl-CoA + chitopentaose 4.7% activity compared to D-glucosamine Rhizomucor miehei ?
-
 ?
acetyl-CoA + chitotetraose 9.3% activity compared to D-glucosamine Rhizomucor miehei ?
-
 ?
acetyl-CoA + chitotriose 12% activity compared to D-glucosamine Rhizomucor miehei ?
-
 ?
acetyl-CoA + D-glucosamine
-
 Rhizomucor miehei CoA + N-acetyl-D-glucosamine
-
 ?
acetyl-CoA + D-glucosamine 100% activity Rhizomucor miehei CoA + N-acetyl-D-glucosamine
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Rhizomucor miehei

Synonyms

Synonyms Comment Organism
GCN5-related glucosamine N-acetyltransferase
-
 Rhizomucor miehei
NAG
-
 Rhizomucor miehei