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Literature summary for 2.3.1.30 extracted from

  • Noji, M.; Saito, K.
    Molecular and biochemical analysis of serine acetyltransferase and cysteine synthase towards sulfur metabolic engineering in plants (2002), Amino Acids, 22, 231-243.
    View publication on PubMed
Search for more literature for 2.3.1.30

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutants in Escherichia coli Citrullus lanatus subsp. vulgaris

Protein Variants

Protein Variants Comment Organism
E278L site-directed mutagenesis, about 1.7fold more sensitive to inhibition by L-cysteine Citrullus lanatus subsp. vulgaris
F285Y site-directed mutagenesis, about 1.7fold less sensitive to inhibition by L-cysteine Citrullus lanatus subsp. vulgaris
G277C site-directed mutagenesis, about 27fold less sensitive to inhibition by L-cysteine Citrullus lanatus subsp. vulgaris
H282Q site-directed mutagenesis, about 3.5fold less sensitive to inhibition by L-cysteine Citrullus lanatus subsp. vulgaris
additional information SAT of transgenic Arabidopsis plants overexpressing the point-mutated watermelon gene are not inhibited by L-cysteine, regardless if the recombinant enzyme is targeted to the chloroplast or expressed in the cytosol Arabidopsis thaliana
additional information SAT of transgenic Arabidopsis plants overexpressing the point-mutated watermelon gene are not inhibited by L-cysteine, regardless if the recombinant enzyme is targeted to the chloroplast or expressed in the cytosol Citrullus lanatus subsp. vulgaris
additional information transgenic Arabidopsis plants expressing recombinant wild-type SATase are inhibited by L-cysteine Arabidopsis thaliana
additional information transgenic Arabidopsis plants expressing recombinant wild-type SATase are inhibited by L-cysteine Citrullus lanatus subsp. vulgaris
S279T site-directed mutagenesis, as sensitive to inhibition by L-cysteine as the wild-type Citrullus lanatus subsp. vulgaris

Inhibitors

Inhibitors Comment Organism Structure
L-cysteine feed-back inhibition; weak Allium tuberosum
L-cysteine feed-back inhibition of cytosolic isozyme SAT-c, not of mitochondrial and plastidic isozymes SAT-m and SAT-p Arabidopsis thaliana
L-cysteine feed-back inhibition Citrullus lanatus subsp. vulgaris
L-cysteine feed-back inhibition Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Arabidopsis thaliana
additional information
-
 additional information Km for L-serine and acetyl-CoA of recombinant L-cysteine-binding motif mutants, overview Citrullus lanatus subsp. vulgaris
0.13
-
 acetyl-CoA
-
 Arabidopsis thaliana
0.13
-
 acetyl-CoA
-
 Citrullus lanatus subsp. vulgaris
0.59
-
 L-serine
-
 Arabidopsis thaliana
0.59
-
 L-serine
-
 Citrullus lanatus subsp. vulgaris

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Spinacia oleracea 9507
-
chloroplast
-
 Arabidopsis thaliana 9507
-
chloroplast
-
 Citrullus lanatus subsp. vulgaris 9507
-
chloroplast
-
 Allium tuberosum 9507
-
cytosol
-
 Spinacia oleracea 5829
-
cytosol
-
 Citrullus lanatus subsp. vulgaris 5829
-
cytosol
-
 Allium tuberosum 5829
-
cytosol isozyme SAT-c Arabidopsis thaliana 5829
-
mitochondrion
-
 Citrullus lanatus subsp. vulgaris 5739
-
mitochondrion
-
 Allium tuberosum 5739
-
mitochondrion isozyme SAT-m, matrix Arabidopsis thaliana 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + L-serine Spinacia oleracea key role in the sulfur assimilatory pathway CoA + O-acetyl-L-serine
-
 ir
acetyl-CoA + L-serine Arabidopsis thaliana key role in the sulfur assimilatory pathway CoA + O-acetyl-L-serine
-
 ir
acetyl-CoA + L-serine Citrullus lanatus subsp. vulgaris key role in the sulfur assimilatory pathway CoA + O-acetyl-L-serine
-
 ir
acetyl-CoA + L-serine Allium tuberosum key role in the sulfur assimilatory pathway CoA + O-acetyl-L-serine
-
 ir

Organism

Organism UniProt Comment Textmining
Allium tuberosum
-
 Chinese chive
-
Arabidopsis thaliana
-
 3 isozymes specific for subcellular compartment: SAT-c, SAT-m, SAT-p
-
Citrullus lanatus subsp. vulgaris
-
-
-
Spinacia oleracea
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + L-serine
-
 Spinacia oleracea CoA + O-acetyl-L-serine
-
 ir
acetyl-CoA + L-serine
-
 Arabidopsis thaliana CoA + O-acetyl-L-serine
-
 ir
acetyl-CoA + L-serine
-
 Citrullus lanatus subsp. vulgaris CoA + O-acetyl-L-serine
-
 ir
acetyl-CoA + L-serine
-
 Allium tuberosum CoA + O-acetyl-L-serine
-
 ir
acetyl-CoA + L-serine key role in the sulfur assimilatory pathway Spinacia oleracea CoA + O-acetyl-L-serine
-
 ir
acetyl-CoA + L-serine key role in the sulfur assimilatory pathway Arabidopsis thaliana CoA + O-acetyl-L-serine
-
 ir
acetyl-CoA + L-serine key role in the sulfur assimilatory pathway Citrullus lanatus subsp. vulgaris CoA + O-acetyl-L-serine
-
 ir
acetyl-CoA + L-serine key role in the sulfur assimilatory pathway Allium tuberosum CoA + O-acetyl-L-serine
-
 ir