Cloned (Comment) | Organism |
---|---|
PCR-amplification, plasmid transferred to Agrobacterium tumefaciens Ag10 for transfection of Lupinus angustifolius (cultivar Kalya) | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
biotechnology | 5fold increase of O-acetylserine concentration and 26fold increase compared to controls of free cystein in transgenic developing narrow leaf lupin embryos (Lupinus angustifolius) without effect on free methionine levels in developing embryos or total cysteine and methionine concentrations in mature seeds | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + acetyl-CoA | Arabidopsis thaliana | - |
O-acetyl-L-serine + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q42588 | - |
- |
Purification (Comment) | Organism |
---|---|
protein extraction from frozen, homogenized embryos | Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
recombinant Arabidopsis serine acetlytransferase activity in transgenic Lupinus angustifolius embryos of the T2 and T4 plant generation is increased severalfold over controls (wild-type and transgenic sunflower seed albumin lupins), 5-12fold and about 5fold in 5F1 (in vitro 4fold over wild-type), 24-31fold and about 20fold in 3A3 (in vitro 12fold over wild-type), 49fold and about 40fold in 5B3 (in vitro 30fold over wild-type), control enzyme activity is roughly about 5 nmol/min/mg protein, correlation to the concentration of key intermediates of the sulphur amino acid biosynthesis (O-acetylserine, free cysteine, glutathione) | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + acetyl-CoA | - |
Arabidopsis thaliana | O-acetyl-L-serine + CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AtSAT1 | - |
Arabidopsis thaliana |
AtSerat 2;1 | - |
Arabidopsis thaliana |
SAT | - |
Arabidopsis thaliana |
SAT-1 | - |
Arabidopsis thaliana |
serine acetlytransferase | - |
Arabidopsis thaliana |