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Literature summary for 2.3.1.30 extracted from

  • Krueger, S.; Niehl, A.; Lopez Martin, M.C.; Steinhauser, D.; Donath, A.; Hildebrandt, T.; Romero, L.C.; Hoefgen, R.; Gotor, C.; Hesse, H.
    Analysis of cytosolic and plastidic serine acetyltransferase mutants and subcellular metabolite distributions suggests interplay of the cellular compartments for cysteine biosynthesis in Arabidopsis (2009), Plant Cell Environ., 32, 349-367.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information T-DNA insertion mutants lead to complete disruption of the respective enzyme genes, mutants for cytosolic (csat) and plastidic (psat) enzyme isoforms, and double mutants (dmsat) of the 2 isoforms by crossing Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
cysteine feedback inhibition; feedback inhibition Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol SERAT1.1 Arabidopsis thaliana 5829
-
mitochondrion compartment with largest serine acetyltransferase activity of total serine acetyltransferase activity independent of cytosolic serine acetyltransferase activity Arabidopsis thaliana 5739
-
mitochondrion compartment with largest serine acetyltransferase activity of total serine acetyltransferase activity independent of plastidic serine acetyltransferase Arabidopsis thaliana 5739
-
plastid SERAT2.1 Arabidopsis thaliana 9536
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-serine + acetyl-CoA Arabidopsis thaliana no major contribution to total cysteine biosynthesis O-acetylserine + CoA
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q42538 At5g56760, SERAT1.1; T-DNA insertion mutants for cytosolic serine acetyltransferase (csat) and plastidic serine acetyltransferase (psat), double mutants of both isoforms (dmsat), and Columbia-0 wild-type
-
Arabidopsis thaliana Q42588 At1g55920 SERAT2.1; T-DNA insertion mutants for cytosolic serine acetyltransferase (csat) and plastidic serine acetyltransferase (psat), double mutants of both isoforms (dmsat), and Columbia-0 wild-type
-

Purification (Commentary)

Purification (Comment) Organism
frozen plant material is homogenized in extraction buffer (50 mM HEPES/KOH, pH 7.4) and centrifuged, supernatant desalted and used for enzyme assays Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
50 mM Tris-HCl, pH 7.5, csat mutant (deficient in cytosolic enzyme), total serine acetyltransferase activity reduced to 71% compared to wild-type in leaves, to 68% in roots Arabidopsis thaliana
additional information
-
50 mM Tris-HCl, pH 7.5, dmsat mutant (deficient in cytosolic and plastidic enzyme), total serine acetyltransferase activity reduced to 80% compared to wild-type in roots, no significant reduction in leaves Arabidopsis thaliana
additional information
-
50 mM Tris-HCl, pH 7.5, psat mutant (deficient in plastidic enzyme), without significant total serine acetyltransferase alterations in leaves and roots Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine + acetyl-CoA no major contribution to total cysteine biosynthesis Arabidopsis thaliana O-acetylserine + CoA
-
?

Synonyms

Synonyms Comment Organism
cytosolic serine acetyltransferase
-
Arabidopsis thaliana
plastidic serine acetyltransferase
-
Arabidopsis thaliana
SAT
-
Arabidopsis thaliana
SERAT1.1
-
Arabidopsis thaliana
SERAT2.1
-
Arabidopsis thaliana
serine acetyltransferase
-
Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana T-DNA insertion mutants lead to complete expression disruption of the respective enzyme genes and decreases in O-acetyl serine and cysteine contents in leaves and roots (cytosolic enzyme and double mutant), a significant gamma-glutamylcysteine decrease in double mutant leaves and cytosolic enzyme mutant roots, a reduction of glutathione content in cytosolic enzyme mutant roots, and sulfate reduction in cytosolic enzyme mutant leaves down
Arabidopsis thaliana T-DNA insertion mutants lead to complete expression disruption of the respective enzyme genes and decreases in O-acetyl serine and cysteine contents in leaves and roots (double mutant), a significant gamma-glutamylcysteine decrease in double mutant leaves, and sulfide reduction in plastidic enzyme mutant leaves down

General Information

General Information Comment Organism
metabolism rate-limiting step in the cysteine biosynthesis Arabidopsis thaliana