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Literature summary for 2.3.1.31 extracted from

  • Miyajima, R.; Shiio, I.
    Regulation of aspartate family amino acid biosynthesis in Brevibacterium flavum. VII. Properities of homoserine O-transacetylase (1973), J. Biochem., 73, 1061-1068.
    View publication on PubMed
Search for more literature for 2.3.1.31

Protein Variants

Protein Variants Comment Organism
additional information methionine auxotroph strains show no activity [Brevibacterium] flavum

Inhibitors

Inhibitors Comment Organism Structure
L-homocysteine not [Brevibacterium] flavum
L-methionine no inhibition [Brevibacterium] flavum
additional information effects of several amino acids with and without addition of L-methionine, overview [Brevibacterium] flavum
O-acetyl-L-homoserine
-
 [Brevibacterium] flavum
S-adenosyl-L-methionine weak [Brevibacterium] flavum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 acetyl-CoA
-
 [Brevibacterium] flavum
2.8
-
 L-homoserine
-
 [Brevibacterium] flavum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + L-homoserine [Brevibacterium] flavum first reaction in L-methionine biosynthesis CoA + O-acetyl-L-homoserine
-
 ?

Organism

Organism UniProt Comment Textmining
[Brevibacterium] flavum
-
 mutant strain M-116 derived from wild-type strain 2247
-

Purification (Commentary)

Purification (Comment) Organism
partial [Brevibacterium] flavum

Reaction

Reaction Comment Organism Reaction ID
acetyl-CoA + L-homoserine = CoA + O-acetyl-L-homoserine ping-pong reaction mechanism [Brevibacterium] flavum
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.143
-
-
 [Brevibacterium] flavum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + L-homoserine succinyl-CoA cannot replace acetyl-CoA [Brevibacterium] flavum CoA + O-acetyl-L-homoserine
-
 ?
acetyl-CoA + L-homoserine first reaction in L-methionine biosynthesis [Brevibacterium] flavum CoA + O-acetyl-L-homoserine
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at [Brevibacterium] flavum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 [Brevibacterium] flavum