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Literature summary for 2.3.1.31 extracted from

  • Nazi, I.; Scott, A.; Sham, A.; Rossi, L.; Williamson, P.R.; Kronstad, J.W.; Wright, G.D.
    Role of homoserine transacetylase as a new target for antifungal agents (2007), Antimicrob. Agents Chemother., 51, 1731-1736.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.31

Application

Application Comment Organism
medicine using a mouse inhalation model of infection it is shown that MET2 is required for virulence, making fungal HTA a viable target for new antibiotic discovery Cryptococcus neoformans

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Cryptococcus neoformans

Protein Variants

Protein Variants Comment Organism
additional information Met auxotrophy is shown by a constructed MET2 mutant and its growth behavior in Met-deficient media Cryptococcus neoformans

Inhibitors

Inhibitors Comment Organism Structure
6-carbamoyl-3a,4,5,9b-tetrahydro-3H-cyclopenta[c]quinoline-4-carboxylic acid competitive inhibitor of acetyl-CoA Cryptococcus neoformans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kcat/Km (substrate L-homoserine): 130000/Msec, (substrate acetyl-CoA): 878000/Msec Cryptococcus neoformans

Organism

Organism UniProt Comment Textmining
Cryptococcus neoformans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Cryptococcus neoformans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + L-homoserine
-
 Cryptococcus neoformans CoA + O-acetyl-L-homoserine
-
 ?

Synonyms

Synonyms Comment Organism
CnHTA
-
 Cryptococcus neoformans
homoserine transacetylase
-
 Cryptococcus neoformans
MET2
-
 Cryptococcus neoformans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
122
-
 L-homoserine
-
 Cryptococcus neoformans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Cryptococcus neoformans

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0136
-
 6-carbamoyl-3a,4,5,9b-tetrahydro-3H-cyclopenta[c]quinoline-4-carboxylic acid Ki value of competitive inhibition of acetyl-CoA Cryptococcus neoformans
0.0917
-
 6-carbamoyl-3a,4,5,9b-tetrahydro-3H-cyclopenta[c]quinoline-4-carboxylic acid Ki value of noncompetitive inhibition of L-homoserine Cryptococcus neoformans

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.156
-
 in the presence of 0.01 mM acetyl-CoA Cryptococcus neoformans 6-carbamoyl-3a,4,5,9b-tetrahydro-3H-cyclopenta[c]quinoline-4-carboxylic acid
0.287
-
 in the presence of 0.1 mM acetyl-CoA Cryptococcus neoformans 6-carbamoyl-3a,4,5,9b-tetrahydro-3H-cyclopenta[c]quinoline-4-carboxylic acid