Literature summary for 2.3.1.31 extracted from

Wang, M.; Liu, L.; Wang, Y.; Wei, Z.; Zhang, P.; Li, Y.; Jiang, X.; Xu, H.; Gong, W.
Crystal structure of homoserine O-acetyltransferase from Leptospira interrogans (2007), Biochem. Biophys. Res. Commun., 363, 1050-1056.

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structure of HTA from Leptospira interrogans is determined at 2.2 A resolution using selenomethionyl single-wavelength anomalous diffraction method. HTA is modular and consists of two structurally distinct domains: a core alpha/beta domain containing the catalytic site and a helical bundle called the lid domain. Structure fold belongs to alpha/beta hydrolase superfamily with the characteristic catalytic triad residues in the active site. The catalytic His and Ser are both present in two conformations, which may be involved in the catalytic mechanism for acetyl transfer	Leptospira interrogans

Crystallization (Comment)

Organism

crystal structure of HTA from Leptospira interrogans is determined at 2.2 A resolution using selenomethionyl single-wavelength anomalous diffraction method. HTA is modular and consists of two structurally distinct domains: a core alpha/beta domain containing the catalytic site and a helical bundle called the lid domain. Structure fold belongs to alpha/beta hydrolase superfamily with the characteristic catalytic triad residues in the active site. The catalytic His and Ser are both present in two conformations, which may be involved in the catalytic mechanism for acetyl transfer

Leptospira interrogans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.95	-	acetyl-CoA	-	Leptospira interrogans
1.6	-	L-homoserine	-	Leptospira interrogans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40000	-	SDS-PAGE	Leptospira interrogans

Organism

Organism	UniProt	Comment	Textmining
Leptospira interrogans	Q8F4I0	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + L-homoserine	-	Leptospira interrogans	CoA + O-acetyl-L-homoserine	-	?

Subunits

Subunits	Comment	Organism
homodimer	crystal structure	Leptospira interrogans

Synonyms

Synonyms	Comment	Organism
homoserine O-acetyltransferase	-	Leptospira interrogans
HTA	-	Leptospira interrogans

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Release 2023.1 (January 2023)