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Literature summary for 2.3.1.37 extracted from

  • Warnick, G.R.; Burnham, B.F.
    Regulation of prophyrin biosynthesis. Purification and characterization of delta-aminolevulinic acid synthase (1971), J. Biol. Chem., 246, 6880-6885.
    View publication on PubMed
Search for more literature for 2.3.1.37

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol activates Cereibacter sphaeroides

General Stability

General Stability Organism
stable to freezing and thawing Cereibacter sphaeroides

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol slightly at high concentration, 10 mM Cereibacter sphaeroides
dithiothreitol
-
 Cereibacter sphaeroides
hemin
-
 Cereibacter sphaeroides

Metals/Ions

Metals/Ions Comment Organism Structure
potassium ferricyanide activates at 0.5 mM Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 gel filtration Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.17
-
 purified enzyme Cereibacter sphaeroides

Storage Stability

Storage Stability Organism
-15°C, 50 mM phosphate buffer, pH 6.8, 10% glycerol, 1 mM 2-mercaptoethanol, stable for several months Cereibacter sphaeroides
4°C, 50 mM phosphate buffer, pH 6.8, 10% glycerol, 1 mM 2-mercaptoethanol, 10% loss of activity after 2 weeks Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinyl-CoA + glycine absolute requirement for glycine Cereibacter sphaeroides 5-aminolevulinate + CoA + CO2
-
 ?
succinyl-CoA + glycine absolute requirement for succinyl-CoA Cereibacter sphaeroides 5-aminolevulinate + CoA + CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 57000, SDS-PAGE Cereibacter sphaeroides
monomer 1 * 57000, SDS-PAGE Cereibacter sphaeroides

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 60 min, 20% loss of activity with addition of 2-mercaptoethanol and glycerol, 60% loss of activity without addition of 2-mercaptoethanol and glycerol Cereibacter sphaeroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4 7.6
-
 Cereibacter sphaeroides

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate absolute requirement Cereibacter sphaeroides