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Literature summary for 2.3.1.37 extracted from

  • Ferreira, G.C.; Vajapey, U.; Hafez, O.; Hunter, G.A.; Barber, M.J.
    Aminolevulinate synthase: lysine 313 is not essential for binding the pyridoxal phosphate cofactor but is essential for catalysis (1995), Protein Sci., 4, 1001-1006.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression of erythroid-specific isoform ALAS-E and mutants in Escherichia coli Mus musculus

Protein Variants

Protein Variants Comment Organism
K313A site-directed mutagenesis, mutants of erythroid-specific isoform, exchange of active site lysine residue 313, binding of pyridoxal 5'-phosphate and glycine noncovalently, reduced activity, because covalent binding is required Mus musculus
K313G site-directed mutagenesis, mutants of erythroid-specific isoform, exchange of active site lysine residue 313, binding of pyridoxal 5'-phosphate and glycine noncovalently, reduced activity, because covalent binding is required Mus musculus
K313H site-directed mutagenesis, mutants of erythroid-specific isoform, exchange of active site lysine residue 313, binding of pyridoxal 5'-phosphate and glycine noncovalently, reduced activity, because covalent binding is required Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
 gene ALAS-E
-
Mus musculus
-
 erythroid-specific isoform
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and Lys313 mutants from Escherichia coli Mus musculus

Reaction

Reaction Comment Organism Reaction ID
succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 covalent binding of pyridoxal 5'-phosphate and glycine to active site Lys131 is required for optimal activity Mus musculus
a
succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 Tyr121, Asp279, Arg439, and Lys313 are involved in substrate and cofactor binding, mechanism, subunit localisation Mus musculus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinyl-CoA + glycine
-
 Mus musculus 5-aminolevulinate + CoA + CO2
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 erythroid-specific isoform Mus musculus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate required Mus musculus
pyridoxal 5'-phosphate content in ALAS-E wild-type and Lys313 mutants Mus musculus