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Literature summary for 2.3.1.37 extracted from

  • Tan, D.; Harrison, T.; Hunter, G.A.; Ferreira, G.C.
    Role of arginine 439 in substrate binding of 5-aminolevulinate synthase (1998), Biochemistry, 37, 1478-1484.
    View publication on PubMed
Search for more literature for 2.3.1.37

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of His-tagged wild-type and mutants in Escherichia coli Mus musculus

Protein Variants

Protein Variants Comment Organism
R433K active site mutant, 2fold increased activity Mus musculus
R433L active site mutant, similar to the wild-type Mus musculus
R439K active site mutant, 77% activity compared to the wild-type, 9-13fold increased Km for both substrates, 5fold increased dissociation constant for glycine Mus musculus
R439L active site mutant, no activity, 30fold increased dissociation constant for glycine Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 succinyl-CoA recombinant erythroid isoform wild-type Mus musculus
0.0022
-
 succinyl-CoA recombinant erythroid isoform mutant R433K Mus musculus
0.0032
-
 succinyl-CoA recombinant erythroid isoform mutant R433L Mus musculus
0.027
-
 succinyl-CoA recombinant erythroid isoform mutant R439K Mus musculus
11.7
-
 glycine recombinant erythroid isoform Mus musculus
14.4
-
 glycine recombinant erythroid isoform mutant R433K Mus musculus
18.4
-
 glycine recombinant erythroid isoform mutant R433L Mus musculus
103
-
 glycine recombinant erythroid isoform mutant R439K Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
 erythroid-specific isoform
-

Reaction

Reaction Comment Organism Reaction ID
succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 Tyr121, Asp279, Arg439, and Lys313 are involved in substrate and cofactor binding, mechanism, subunit localisation Mus musculus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinyl-CoA + glycine
-
 Mus musculus 5-aminolevulinate + CoA + CO2
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.508
-
 succinyl-CoA recombinant erythroid mutant R439K Mus musculus
0.658
-
 succinyl-CoA recombinant erythroid isoform Mus musculus
0.842
-
 succinyl-CoA recombinant erythroid mutant R433L Mus musculus
1.35
-
 succinyl-CoA recombinant erythroid mutant R433K Mus musculus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate required Mus musculus