Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutants in Escherichia coli BL21 (DE3) | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
R606A | oligonucleotide-directed in vitro mutagenesis, 6.6fold increase in activity about 16000fold increase in selectivity for acetyl-CoA against malonyl-CoA, increased production of medium-chain length fatty acids | Rattus norvegicus |
R606K | oligonucleotide-directed in vitro mutagenesis, 1.7fold increase in activity about 16fold increase in selectivity for acetyl-CoA against malonyl-CoA | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0018 | - |
acetyl-CoA | recombinant purified mutant R606A | Rattus norvegicus | |
0.0029 | - |
acetyl-CoA | recombinant purified mutant R606K | Rattus norvegicus | |
0.0039 | - |
acetyl-CoA | recombinant purified wild-type enzyme | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant mutants from E. coli | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + an [acyl-carrier protein] = CoA + an acetyl-[acyl-carrier protein] | mechanism | Rattus norvegicus | |
acetyl-CoA + an [acyl-carrier protein] = CoA + an acetyl-[acyl-carrier protein] | Arg-606 is responsible for malonyl-CoA specificity of the bifunctional enzyme | Rattus norvegicus |
Renatured (Comment) | Organism |
---|---|
recombinant enzyme purified from E. coli, refolding after treatment of inclusion bodies with urea | Rattus norvegicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
2.68 | - |
recombinant purified wild-type enzyme | Rattus norvegicus |
4.53 | - |
recombinant purified mutant R606K | Rattus norvegicus |
17.8 | - |
recombinant purified mutant R606A | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + pantetheine | dual specificity of a malonyl-CoA/acetyl-CoA:acyl carrier protein S-acyltransferase | Rattus norvegicus | CoA + acetyl-S-pantetheine | - |
r | |
acetyl-CoA + pantetheine | octanoyl-S-pantetheine, decanoyl-S-pantetheine, and dodecanoyl-S-pantetheine can also serve as substrates for the backward reaction | Rattus norvegicus | CoA + acetyl-S-pantetheine | - |
r | |
acetyl-CoA + [acyl-carrier protein] | dual specificity of a malonyl-CoA/acetyl-CoA:acyl carrier protein S-acyltransferase | Rattus norvegicus | CoA + acetyl-[acyl-carrier protein] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
malonyl-CoA/acetyl-CoA:acyl carrier protein S-acyltransferase | - |
Rattus norvegicus |
More | cf. EC 2.3.1.85 and EC 2.3.1.86 | Rattus norvegicus |
More | dual specificity of a malonyl-CoA/acetyl-CoA:acyl carrier protein S-acyltransferase | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
acetyl-CoA | recombinant purified wild-type enzyme | Rattus norvegicus | |
3.4 | - |
acetyl-CoA | recombinant purified mutant R606K | Rattus norvegicus | |
4.2 | - |
acetyl-CoA | recombinant purified mutant R606A | Rattus norvegicus |