Literature summary for 2.3.1.39 extracted from

Kremer, L.; Nampoothiri, K.M.; Lesjean, S.; Dover, L.G.; Graham, S.; Betts, J.; Brennan, P.J.; Minnikin, D.E.; Locht, C.; Besra, G.S.
Biochemical characterization of acyl carrier protein (AcpM) and malonyl-CoA:AcpM transacylase (mtFabD), two major components of Mycobacterium tuberculosis fatty acid synthase II (2001), J. Biol. Chem., 276, 27967-27974.

Search for more literature for 2.3.1.39

Cloned(Commentary)

Cloned (Comment)	Organism
a hexa-histidine-tagged enzyme overexpressed in Escherichia coli	Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0126	-	malonyl-CoA	recombinant enzyme	Mycobacterium tuberculosis
0.0141	-	holo-acyl-carrier protein	holo-acyl-carrier protein from Mycobacterium tuberculosis	Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
30700	-	calculated from amino acid sequence, consistent with the observed molecular mass of approximately 33000 Da for the enzyme containing the 6-His N-terminal extension	Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+-chelate chromatography	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
malonyl-CoA + acyl-carrier protein	holo-acyl-carrier-protein from Mycobacterium tuberculosis constitutes the preferred substrate for the enzyme, however the enzyme uses both E. coli holo-acyl-carrier-protein and Mycobacterium tuberculosis holo-acyl-carrier-protein as substrates for transacylation in vivo and in vitro	Mycobacterium tuberculosis	CoA + malonyl-[acyl-carrier protein]	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.8	-	assay at	Mycobacterium tuberculosis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)