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Literature summary for 2.3.1.4 extracted from
- Purification of glucosamine-6-phosphate N-acetylase from sheep brain (1962), Biochim. Biophys. Acta, 59, 681-689.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | activates | Ovis aries |  |
| free sulfhydryl groups | activate | Ovis aries | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | high concentrations | Ovis aries | |
| EDTA | - |
Neurospora crassa | |
| Ni2+ | - |
Ovis aries | |
| p-chloromercuribenzoate | - |
Neurospora crassa | |
| p-chloromercuribenzoate | 0.001 mM, 50% inhibition, 0.01 mM, complete inhibition, reversed by cysteine | Ovis aries | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
acetyl-CoA | - |
Ovis aries | |
| 0.45 | - |
D-glucosamine 6-phosphate | - |
Ovis aries | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | slight but consistent enhancing effect | Ovis aries | |
| Mn2+ | slight but consistent enhancing effect | Ovis aries | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | - |
- |
- |
| Ovis aries | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, calcium phosphate gel, heat treatment at 65°C | Ovis aries |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Ovis aries | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0054 | - |
- |
Ovis aries |
Storage Stability
| Storage Stability | Organism |
|---|---|
| frozen state, 2 weeks, 50% loss of activity | Ovis aries |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + D-glucosamine 6-phosphate | - |
Neurospora crassa | CoA + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
| acetyl-CoA + D-glucosamine 6-phosphate | - |
Ovis aries | CoA + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
| propionyl-CoA + D-glucosamine 6-phosphate | 15% of activity with acetyl-CoA | Ovis aries | CoA + N-propionyl-D-glucosamine 6-phosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.1 | - |
Neurospora crassa |
| 7.4 | - |
- |
Ovis aries |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | approx. 90% of maximal activity at pH 6.0, approx. 95% of maximal activity at pH 8.0 | Neurospora crassa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no cofactor required | Neurospora crassa |
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Release 2023.1 (January 2023)
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