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Literature summary for 2.3.1.4 extracted from
- An enzyme-coupled assay for amidotransferase activity of glucosamine-6-phosphate synthase (2007), Anal. Biochem., 370, 142-146.
Application
| Application | Comment | Organism |
|---|---|---|
| molecular biology | an assay for glucosamine-6-phosphate synthase using a yeast glucosamine-6-phosphate N-acetyltransferase 1 (GNA1) as coupling enzyme is developed. The assay measures the production of glucosamine-6-phosphate by either following the consumption of acetyl-CoA spectrophotometrically at 230 nm or quantifying the free thiol with 5,50-dithio-bis(2-nitrobenzoic acid) (Ellmans reagent) in a discontinuous manner. This method is simple to perform and can be adapted to a 96-well microtiter plate format | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + D-glucosamine 6-phosphate | - |
Saccharomyces cerevisiae | CoA + N-acetyl-D-glucosamine 6-phosphate | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glucosamine-6-phosphate N-acetyltransferase | - |
Saccharomyces cerevisiae |
| GNA1 | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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