Cloned (Comment) | Organism |
---|---|
expression of His-tagged FabH1 and FabH2 in Escherichia coli INValphaF' | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid sequence alignment | Bacillus subtilis |
additional information | - |
amino acid sequence alignment | Escherichia coli |
39800 | - |
x * 39800, FabH2, SDS-PAGE | Bacillus subtilis |
42700 | - |
x * 42700, FabH1, SDS-PAGE | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | Escherichia coli | - |
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | Bacillus subtilis | initiation of fatty acid biosynthesis with acetyl-CoA as a primer, overview substrate specificity | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
genes fabH1 and fabH2, 2 synthase III isozymes | - |
Escherichia coli | - |
synthase III, gene fabH | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged proteins FabH1 and FabH2 from E. coli | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bacillus subtilis |
additional information | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Escherichia coli | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | initiation of fatty acid biosynthesis with acetyl-CoA as a primer, overview substrate specificity | Bacillus subtilis | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
additional information | - |
Escherichia coli | ? | - |
? | |
additional information | synthase III isozymes also utilize iso- and anteiso-branched-chain acyl-CoA primers as substrates | Bacillus subtilis | ? | - |
? | |
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | substrate specificity, overview | Bacillus subtilis | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | substrate specificity, overview | Escherichia coli | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42700, FabH1, SDS-PAGE | Bacillus subtilis |
? | x * 39800, FabH2, SDS-PAGE | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Bacillus subtilis |
7 | - |
assay at | Escherichia coli |