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Literature summary for 2.3.1.41 extracted from
- Identification and molecular characterization of the beta-ketoacyl-[acyl carrier protein] synthase component of the Arabidopsis mitochondrial fatty acid synthase (2004), J. Biol. Chem., 279, 8242-8251.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| complements KAS II defect in Escherichia coli | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cerulenin | - |
Arabidopsis thaliana |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
x * 47000, SDS-PAGE | Arabidopsis thaliana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q8L3X9 | expression in Escherichia coli | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | 20 amino acid mitochondrial targeting sequence | Arabidopsis thaliana |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Arabidopsis thaliana |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
enzyme from fresh extract synthesizes prominent amounts of C14 and C16 acyl chains. After storage at -20°C for one or several weeks, enzyme synthesizes predominantly C8 acyl chains | Arabidopsis thaliana |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, unstable in crude extracts lacking glycerol. Enzyme from fresh extract synthesizes prominent amounts of C14 and C16 acyl chains. After storage at -20°C for one or several weeks, enzyme synthesizes predominantly C8 acyl chains | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Arabidopsis thaliana | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| additional information | no substrate: acetyl-[acyl-carrier protein] | Arabidopsis thaliana | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 47000, SDS-PAGE | Arabidopsis thaliana |
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Release 2023.1 (January 2023)
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