Literature summary for 2.3.1.41 extracted from

Brown, A.K.; Sridharan, S.; Kremer, L.; Lindenberg, S.; Dover, L.G.; Sacchettini, J.C.; Besra, G.S.
Probing the mechanism of the Mycobacterium tuberculosis beta-ketoacyl-ACP synthase III mtFabH: Factors influencing catalysis and substrate specificity (2005), J. Biol. Chem., 280, 32539-32547.

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Protein Variants

Protein Variants	Comment	Organism
R161A	69.6% of wild-type activity	Mycobacterium tuberculosis
R46A	7.3% of wild-type activity	Mycobacterium tuberculosis
R46A/R161A	0.3% of wild-type activity	Mycobacterium tuberculosis
T97F	no enzymic activity	Mycobacterium tuberculosis
W42A	21.5% of wild-type activity	Mycobacterium tuberculosis
W42A/R161A	0.2% of wild-type activity	Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0023	-	dodecanoyl-[acyl-carrier protein]	pH 7.0, 37°C	Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0017	-	substrate hexanoyl-[acyl-carrier protein], pH 7.0, 37°C	Mycobacterium tuberculosis
0.0055	-	substrate octanoyl-[acyl-carrier protein], pH 7.0, 37°C	Mycobacterium tuberculosis
0.0062	-	substrate decanoyl-[acyl-carrier protein], pH 7.0, 37°C	Mycobacterium tuberculosis
0.0072	-	substrate dodecanoyl-[acyl-carrier protein], pH 7.0, 37°C	Mycobacterium tuberculosis
0.009	-	substrate eicosanoyl-[acyl-carrier-protein], pH 7.0, 37°C	Mycobacterium tuberculosis
0.0093	-	substrate palmitoyl-[acyl-carrier protein], pH 7.0, 37°C	Mycobacterium tuberculosis
0.0095	-	substrate myristoyl-[acyl-carrier protein], pH 7.0, 37°C	Mycobacterium tuberculosis
0.0104	-	substrate octadecanoyl-[acyl-carrier-protein], pH 7.0, 37°C	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
decanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	-	Mycobacterium tuberculosis	dodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]	-	?
dodecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	-	Mycobacterium tuberculosis	3-oxotetradecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]	-	?
eicosanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	-	Mycobacterium tuberculosis	3-oxodocosanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]	-	?
hexanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	-	Mycobacterium tuberculosis	3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]	-	?
additional information	enzyme is able to use Escherichia coli acyl-carrier protein. Enzyme extends longer, physiologically relevant acyl-CoA primers when paired with its native acyl-carrier protein than with Escherichia coli acyl-carrier protein	Mycobacterium tuberculosis	?	-	?
myristoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	-	Mycobacterium tuberculosis	3-oxohexadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]	-	?
octadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	-	Mycobacterium tuberculosis	3-oxoeicosanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]	-	?
octanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	-	Mycobacterium tuberculosis	3-oxodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]	-	?
palmitoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	-	Mycobacterium tuberculosis	3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]	-	?

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Release 2023.1 (January 2023)