Application | Comment | Organism |
---|---|---|
drug development | the enzyme is a target for antibacterial drugs | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of the His-tagged enzyme in Escherichia coli strains XL-1 blue and M15 | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme free or complexed with acyl-CoA, hanging drop vapour diffusion method, 0.002 ml of protein solution, containing 0.087 mg of protein with or without 4 mM hexanoyl-CoA, mixed with 0.002 ml of reservoir solution containing 24% w/v PEG 3350 and 0.2 M NH4Cl, 5-8 days at room temperature, rod-shaped single crystals, X-ray diffraction structure determination and analysis at 1.6 A resolution, structure modeling | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cerulenin | - |
Homo sapiens | |
additional information | inhibitor binding structure determination | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | beta-ketoacyl [ACP] synthase from the mitochondrial type II fatty acid synthase | Homo sapiens | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9NWU1 | the human beta-ketoacyl [ACP] synthase from the mitochondrial type II fatty acid synthase | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strains XL-1 blue and M15 | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] | structure-activity analysis, reaction mechanism | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | KAS catalyzes the C2 fatty acid elongation reaction using a Cys-His-His catalytic triad | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | secondary structure, a dimer with an alpha-beta-alpha-beta-alpha-thiolase fold capped by an alpha-helical region connecting the strands of the N-terminal beta-sheet, crystal structure analysis | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
beta-ketoacyl [ACP] synthase | - |
Homo sapiens |
beta-ketoacyl-ACP synthase | - |
Homo sapiens |
KAS | - |
Homo sapiens |