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Literature summary for 2.3.1.41 extracted from

  • Borgaro, J.; Chang, A.; MacHutta, C.; Zhang, X.; Tonge, P.
    Substrate recognition by beta-ketoacyl-ACP synthases (2011), Biochemistry, 50, 10678-10686.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.41

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged protein expressed in Escherichia coli pLysS Escherichia coli
His-tagged proteinsexpressed in Mycobacterium smegmatis mc2155 Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
C171Q mimics the acyl enzyme intermediate Mycobacterium tuberculosis
K151Q conserved between FabB, FabF and KasA, proposed to be important for ACP recognition Escherichia coli
K63Q conserved between FabB, FabF and KasA, proposed to be important for ACP recognition Escherichia coli
R62Q conserved between FabB, FabF and KasA, proposed to be important for ACP recognition Escherichia coli
R66Q conserved between FabB, FabF and KasA, proposed to be important for ACP recognition Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
palmitoyl-CoA substrate inhibition above 0.003 mM Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
 palmitoyl-CoA acceptor malonyl-ACP, 0.01 mM malonyl-ACP, pH 8.5, 25°C Mycobacterium tuberculosis
0.0032
-
 lauroyl-ACP wild type protein, acceptor malonyl-ACP, 0.01 mM malonyl-ACP, pH 6.5, 25°C Escherichia coli
0.0039
-
 lauroyl-ACP wild type protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli
0.0047
-
 malonyl-phosphopantetheine-8-mer donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25°C Mycobacterium tuberculosis
0.0051
-
 malonyl-phosphopantetheine-16-mer donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25°C Mycobacterium tuberculosis
0.0058
-
 malonyl-ACP donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25°C Mycobacterium tuberculosis
0.0067
-
 lauroyl-ACP K151Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli
0.0086
-
 malonyl-phosphopantetheine-14-mer donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25°C Mycobacterium tuberculosis
0.009
-
 malonyl-CoA donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25°C Mycobacterium tuberculosis
0.0115
-
 malonyl-ACP wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
0.0142
-
 malonyl-phosphopantetheine-8-mer wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
0.0158
-
 malonyl-phosphopantetheine-14-mer wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
0.029
-
 malonyl-phosphopantetheine-16-mer wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
0.05
-
 lauroyl-ACP R62Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli
0.056
-
 lauroyl-ACP R66Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli
0.0586
-
 lauroyl-CoA wild type protein, acceptor malonyl-ACP, 0.01 mM malonyl-ACP, pH 6.5, 25°C Escherichia coli
0.06
-
 lauroyl-ACP K63Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli
0.153
-
 malonyl-CoA wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography (Ni2+) Escherichia coli
immobilized metal ion affinity chromatography (Ni2+) Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-ACP + lauroyl-ACP
-
 Escherichia coli ?
-
 ?
malonyl-ACP + lauroyl-CoA
-
 Escherichia coli ?
-
 ?
malonyl-ACP + palmitoyl-ACP
-
 Mycobacterium tuberculosis ?
-
 ?
malonyl-ACP + palmitoyl-CoA
-
 Mycobacterium tuberculosis ?
-
 ?
malonyl-CoA + lauroyl-ACP
-
 Escherichia coli ?
-
 ?
malonyl-CoA + palmitoyl-ACP
-
 Mycobacterium tuberculosis ?
-
 ?
malonyl-phosphopantetheine-14-mer + lauroyl-ACP
-
 Escherichia coli ?
-
 ?
malonyl-phosphopantetheine-14-mer + palmitoyl-ACP
-
 Mycobacterium tuberculosis ?
-
 ?
malonyl-phosphopantetheine-16-mer + lauroyl-ACP
-
 Escherichia coli ?
-
 ?
malonyl-phosphopantetheine-16-mer + palmitoyl-ACP
-
 Mycobacterium tuberculosis ?
-
 ?
malonyl-phosphopantetheine-8-mer + lauroyl-ACP
-
 Escherichia coli ?
-
 ?
malonyl-phosphopantetheine-8-mer + palmitoyl-ACP
-
 Mycobacterium tuberculosis ?
-
 ?

Synonyms

Synonyms Comment Organism
beta-ketoacyl-ACP synthase I
-
 Escherichia coli
beta-ketoacyl-ACP synthase I
-
 Mycobacterium tuberculosis
FabB
-
 Escherichia coli
KasA
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.011
-
 malonyl-ACP wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
0.018
-
 malonyl-phosphopantetheine-8-mer wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
0.025
-
 lauroyl-ACP K151Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli
0.033
-
 lauroyl-ACP wild type protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli
0.04
-
 malonyl-phosphopantetheine-14-mer wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
0.053
-
 lauroyl-ACP R66Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli
0.057
-
 lauroyl-ACP wild type protein, acceptor malonyl-ACP, 0.01 mM malonyl-ACP, pH 6.5, 25°C Escherichia coli
0.073
-
 lauroyl-CoA wild type protein, acceptor malonyl-ACP, 0.01 mM malonyl-ACP, pH 6.5, 25°C Escherichia coli
0.077
-
 lauroyl-ACP R62Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli
0.08
-
 palmitoyl-CoA acceptor malonyl-ACP, 0.01 mM malonyl-ACP, pH 8.5, 25°C Mycobacterium tuberculosis
0.083
-
 lauroyl-ACP K63Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli
0.11
-
 malonyl-phosphopantetheine-16-mer wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
0.12
-
 malonyl-CoA wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
0.3
-
 malonyl-phosphopantetheine-16-mer donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25°C Mycobacterium tuberculosis
0.35
-
 malonyl-CoA donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25°C Mycobacterium tuberculosis
0.35
-
 malonyl-phosphopantetheine-8-mer donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25°C Mycobacterium tuberculosis
0.38
-
 malonyl-phosphopantetheine-14-mer donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25°C Mycobacterium tuberculosis
0.47
-
 malonyl-ACP donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25°C Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.78
-
 malonyl-CoA wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
0.83
-
 lauroyl-ACP R66Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli
1.2
-
 lauroyl-ACP K63Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli
1.27
-
 lauroyl-CoA wild type protein, acceptor malonyl-ACP, 0.01 mM malonyl-ACP, pH 6.5, 25°C Escherichia coli
1.3
-
 malonyl-phosphopantetheine-8-mer wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
1.5
-
 lauroyl-ACP R62Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli
3
-
 malonyl-phosphopantetheine-14-mer wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
3.7
-
 lauroyl-ACP K151Q mutant protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli
3.8
-
 malonyl-phosphopantetheine-16-mer wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
8.3
-
 lauroyl-ACP wild type protein, acceptor malonyl-CoA, 0.2 mM malonyl-CoA, pH 6.5, 25°C Escherichia coli
10
-
 malonyl-ACP wild type protein, donor lauroyl-ACP, 0.01 mM lauroyl-ACP, pH 6.5, 25°C Escherichia coli
18.3
-
 lauroyl-ACP wild type protein, acceptor malonyl-ACP, 0.01 mM malonyl-ACP, pH 6.5, 25°C Escherichia coli
38.3
-
 malonyl-CoA donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25°C Mycobacterium tuberculosis
48.3
-
 malonyl-phosphopantetheine-14-mer donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25°C Mycobacterium tuberculosis
60
-
 malonyl-phosphopantetheine-16-mer donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25°C Mycobacterium tuberculosis
75
-
 malonyl-phosphopantetheine-8-mer donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25°C Mycobacterium tuberculosis
86.7
-
 malonyl-ACP donor palmitoyl-ACP, 0.012 mM palmitoyl-ACP, pH 8.5, 25°C Mycobacterium tuberculosis