Cloned (Comment) | Organism |
---|---|
expression in Chinese hamster ovary cells, recombinant enzyme is structurally similar to plasma LCAT | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
mass spectrometry analysis gives a range from 55700 to 59600 | Homo sapiens |
49000 | - |
x * 49000, denatured and deglycosylated enzyme | Homo sapiens |
73000 | - |
x * 73000, recombinant enzyme, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | glycosylation in a recombinant enzyme is more extensive than that of isolated from plasma | Homo sapiens |
Purification (Comment) | Organism |
---|---|
purification of recombinant enzymes | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
recombinant enzyme is half as enzymatically active as plasma LCAT | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylcholine + sterol | - |
Homo sapiens | 1-acylglycerophosphocholine + sterol ester | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 73000, recombinant enzyme, SDS-PAGE | Homo sapiens |
? | x * 49000, denatured and deglycosylated enzyme | Homo sapiens |